interests = My interest is a signal transduction pathway toward the branching of Polysphondulium palladium. We found that under the light. P. palladium of which a branching(Bra) gene was knockout started early in its branching. WT started normally at T22. On the other hand, under the dark WT develops very lately, in contrast Brad- begins to branch at T28. We conclude that the Br gene plays a role in regulation.