dictyNews Electronic Edition Volume 33, number 9 October 9, 2009 Please submit abstracts of your papers as soon as they have been accepted for publication by sending them to dicty@northwestern.edu or by using the form at http://dictybase.org/db/cgi-bin/dictyBase/abstract_submit. Back issues of dictyNews, the Dicty Reference database and other useful information is available at dictyBase - http://dictybase.org. Follow dictyBase now on twitter: http://twitter.com/dictybase ========= Abstracts ========= Dynamics of the Dictyostelium discoideum mitochondrial proteome during vegetative growth, starvation and early stages of development Malgorzata Czarna 1,2,§, Gregory Mathy 1, Allan Mac’Cord 1, Rowan Dobson 3, Wieslawa Jarmuszkiewicz 2, Claudine M. Sluse-Goffart 1, Pierre Leprince 4, Edwin De Pauw 3 and Francis E. Sluse 1 1 Laboratory of Bioenergetics and Cellular Physiology, University of Liege, Belgium 2 Laboratory of Bioenergetics, Adam Mickiewicz University, Poznan, Poland 3 Laboratory of Mass Spectrometry, University of Liege, Belgium 4 GIGA-Neuroscience, University of Liege, Belgium § Present address: Institute of Microbiology, Free University of Berlin, Germany, E-mail: m.czarna@fu-berlin.de Proteomics, in press In this study a quantitative comparative proteomics approach has been used to analyze the D. discoideum mitochondrial proteome variations during vegetative growth, starvation and the early stages of development. Application of 2D-DIGE technology allowed the detection of around 2000 protein spots on each two-dimensional gel with 180 proteins exhibiting significant changes in their expression level. In total, 96 proteins (51 unique and 45 redundant) were unambiguously identified. We show that the D. discoideum mitochondrial proteome adaptations mainly affect energy metabolism enzymes (the Krebs cycle, anaplerotic pathways, the oxidative phosphorylation system and energy dissipation), proteins involved in developmental and signalling processes as well as in protein biosynthesis and fate. The most striking observations were the opposite regulation of expression of citrate synthase and aconitase and the very large variation in the expression of the alternative oxidase (AOX) that highlighted the importance of citrate and AOX in the physiology of the development of D. discoideum. Mitochondrial energy states measured in vivo with MitoTracker Orange CMTMRos showed an increase in mitochondrial membrane polarisation during D. discoideum starvation and starvation-induced development. Submitted by Malgorzata Czarna [m.czarna@fu-berlin.de] ============================================================== [End dictyNews, volume 33, number 9]