dictyNews Electronic Edition Volume 41, number 17 Sugust 14, 2015 Please submit abstracts of your papers as soon as they have been accepted for publication by using the form at http://dictybase.org/db/cgi-bin/dictyBase/abstract_submit. Back issues of dictyNews, the Dicty Reference database and other useful information is available at dictyBase - http://dictybase.org. Follow dictyBase on twitter: http://twitter.com/dictybase ========= Abstracts ========= Proximity-dependent biotin identification (BioID) in Dictyostelium amoebae Petros Batsios, Irene Meyer and Ralph Gräf Institute for Biochemistry and Biology, Dept. of Cell Biology, University of Potsdam, D-14476 Potsdam-Golm, Karl-Liebknecht-Str. 24-25, Methods in Enzymology, in press The identification of a bona fide lamin-like protein in Dictyostelium made this lower eukaryote an attractive model organism to study evolutionarily conserved nuclear envelope (NE) proteins important for nuclear organization and human laminopathies. Proximity-dependent biotin identification (BioID), reported by Roux and colleagues, is a powerful discovery tool for lamin-associated proteins. In this method, living cells express a bait protein (e.g., lamin) fused to an R118G-mutated version of BirA, an E. coli biotinylase. In the presence of biotin, BirA-R118G biotinylates target proteins in close proximity in vivo, which are purified using streptavidin and identified by immunoblotting or mass spectrometry. We adapted the BioID method for use in Dictyostelium amoebae. The protocols described here successfully revealed Dictyostelium lamin-like protein NE81 proximity to Sun1, a conserved inner nuclear membrane protein. Submitted by Ralph Graef [rgraef@uni-potsdam.de] ============================================================== [End dictyNews, volume 41, number 17]