Dicty_Life - A Mutant Phenotype Database for Dictyostelium

Total Number of Entries:4091
Number of Distinct Strains:2606

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Use the search function in the following way. If, by way of example, you wish to know how many of the life cycle movies have defective waves, simply click in the boxes Aberrant and waves, then search.




This will return 4 pages of data (as of 1 August 2007).





Ignore the Strain and Internal ID columns. The interesting results are first, the brief annotation columns - Gwth (growth), Wave (wave propagation patterns), Strm (streaming phenotype), Mund (wild-type or mutant mound formation), Slug (slug phenotype), and Culm (culmination phenotype) -and second, the video clips of the life cycle (click on the column Movies).





Here you will find a video clip of the raw data for wave propagation gathered by dark-field microscopy (original part 1), frame subtracted clips based on this data set (frame-subtracted), and a video clip of bright-field images documenting the life cycle from late streaming through the mound to the slug stage (original part 2). Note that not all video records are complete). Finally, there is a wavelet portrait, a quantitative representation of wave propagation. In brief, this is a plot of wave frequency vs. time in minutes, with the wave amplitude coded in false color (See Sawai et al for a full description). Try comparing a WT and mutant portrait to get a feeling for the usefulness of this representation e.g. compare AX4#293 to rdeA.

Note that the fourth column allows the user to link directly to the annotated gene in dictyBase. when the REMI insertion falls within a gene.