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Gupta, Neha, Saran, Shweta, (2018) ' Deletion of etoposide-induced 2.4 kb transcript (ei24) reduced cell proliferation and aggregate-size in Dictyostelium discoideum. ' Int. J. Dev. Biol. 62 273-283
  
 
Abstract:The etoposide-induced 2.4 kb transcript (ei24) gene is induced both by p53 and etoposide, an anti-cancer tumour drug. There is no p53 gene present in Dictyostelium discoideum. Thus, the functions of ei24 in the absence of p53 were analysed. Both overexpressor (ei24OE) and knockout (ei24-) mutants were made to study its role during growth, development and differentiation. Additionally, cell cycle and its response to DNA-damage were also analysed. We identified, characterized and elucidated the functions of the ei24 gene in Dictyostelium. In silico analyses demonstrated the conservation across eukaryotes and in situ hybridization showed it to be prestalk-specific. ei24- cells showed reduced cell proliferation and cell-cohesive properties, ultimately forming small-sized aggregates that developed into miniature and stalky fruiting bodies. The ei24OE cells formed fruiting bodies with engorged or double-decker type sori with short stalks. The ei24- cells showed reduced cAMP signalling with lower intracellular cAMP levels resulting in diminished migration of cells along cAMP gradients. Deletion of ei24 resulted in mis-expression of prestalk-specific markers. Cell cycle analysis revealed an increased bias towards the stalk-pathway by ei24- cells and vice-versa for ei24OE cells. EI24 in Dictyostelium functions even in the absence of p53 and is induced in response to both UV-radiation and etoposide treatments. ei24OE cells showed enhanced DNA-damage repair mechanisms. Also, etoposide treatment and overexpression of ei24 caused G2/M arrest in the cell cycle. Our results indicate that EI24 is important for the growth, development and differentiation of Dictyostelium apart from being a DNA-damage response gene.
Status: ppublish Type: Journal article Source: PUBMED PubMed ID: 29877566

 
Genes addressed in this paper
DDB_G0288895 acaA cadA carA-1 carA-2 cotA cotB csaA
Topics in this paper
Mammalian Gene Related







Non-mammalian Gene Related







Cellular Location







Function/Process







RNA Levels and Processing X X X X X X X X
Cell Cycle







Cell Type Localization







Regulatory Role







Protein Physical Properties







Protein Functional Domain







Strains/Constructs







Chemotaxis/Motility







Development/Morphogenesis







Phylogenetic Analysis







Growth







Regulated By
X X X X X X X
Mutants/Phenotypes







Sporulation/Germination









Genes addressed in this paper
ctnA dnapkcs ei24 gbfA ku70 ku80 pdsA rad51-1
Topics in this paper
Mammalian Gene Related

X




Non-mammalian Gene Related

X




Cellular Location

X




Function/Process

X




RNA Levels and Processing X X X X X X X X
Cell Cycle

X




Cell Type Localization

X




Regulatory Role

X




Protein Physical Properties

X




Protein Functional Domain

X




Strains/Constructs

X




Chemotaxis/Motility

X




Development/Morphogenesis

X




Phylogenetic Analysis

X




Growth

X




Regulated By X X
X X X X X
Mutants/Phenotypes

X




Sporulation/Germination

X






Genes addressed in this paper
rad51-2 rad52 rad54
Topics in this paper
Mammalian Gene Related


Non-mammalian Gene Related


Cellular Location


Function/Process


RNA Levels and Processing X X X
Cell Cycle


Cell Type Localization


Regulatory Role


Protein Physical Properties


Protein Functional Domain


Strains/Constructs


Chemotaxis/Motility


Development/Morphogenesis


Phylogenetic Analysis


Growth


Regulated By X X X
Mutants/Phenotypes


Sporulation/Germination