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Marinovic, Maja, Xiong, Huajiang, Rivero, Francisco, Weber, Igor, (2018) ' Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum. ' Methods Mol. Biol. 1821 371-392
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Abstract:The model organism D. discoideum is well suited to investigate basic questions of molecular and cell biology, particularly those related to the structure, regulation, and dynamics of the cytoskeleton, signal transduction, cell-cell adhesion, and development. D. discoideum cells make use of Rho-regulated signaling pathways to reorganize the actin cytoskeleton during chemotaxis, endocytosis, and cytokinesis. In this organism the Rho family encompasses 20 members, several belonging to the Rac subfamily, but there are no representatives of the Cdc42 and Rho subfamilies. Here we present protocols suitable for monitoring the actin polymerization response and the activation of Rac upon stimulation of aggregation-competent cells with the chemoattractant cAMP, and for monitoring the localization and dynamics of Rac activity in live cells.
Status: ppublish Type: Journal article Source: PUBMED PubMed ID: 30062425

Genes addressed in this paper
rac1A rac1B rac1C racA racB racF1 racF2
Topics in this paper
Techniques and Reagents X X X X X X X