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Sharma, Priyanka, Kumar, Rishikesh, Saran, Shweta, (2018) ' Overexpression of S-adenosylmethionine decarboxylase impacts polyamine homeostasis during development of Dictyostelium discoideum. ' Int. J. Dev. Biol. 62 647-652
  
 
Abstract:The polyamines putrescine, spermidine and spermine are essential polycations involved in the regulation of cellular proliferation. They exert dynamic effects on nucleic acids and macromolecular synthesis in vitro but their specific functions in vivo are poorly understood. Here, we have modulated the spermidine levels either by overexpressing the S-adenosylmethionine decarboxylase (samdc) gene or treating the cells with methylglyoxal-bis (guanylhydrazone) (MGBG), an inhibitor of SAMDC. In Dictyostelium, overexpression of SAMDC slowed cell proliferation, delayed development and arrested cells in the S-phase of the cell cycle. Treatment with MGBG reduced cell proliferation and stimulated development, but in samdcOE cells, it increased cell proliferation suggesting critical levels of spermidine to be important. In samdcOE cells, spermidine levels remained high throughout development but only small changes in the spermine levels were observed. Initial putrescine levels did increase but reverted to wild-type levels after the mound stage. As tight regulation of polyamine homeostasis is required, we identified genes that could be involved in its maintenance. In conclusion, we characterised samdcOE cells and observed the maintenance of polyamine homeostasis during the development of Dictyostelium cells.
Status: ppublish Type: Journal article Source: PUBMED PubMed ID: 30378390

 
Genes addressed in this paper
DDB_G0279851 DDB_G0287303 amd1 ctrA ctrB ctrC maoA spsA
Topics in this paper
Mammalian Gene Related

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Cellular Location

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Synonym

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Function/Process

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Cell Cycle

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RNA Levels and Processing

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Transcriptional Regulation X X
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Regulatory Role

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Protein Physical Properties X X X X X X X X
Protein Functional Domain X X X X X X X X
Strains/Constructs

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Development/Morphogenesis

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Phylogenetic Analysis X X X X X X X X
Regulated By X X
X X X X X
Growth

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Mutants/Phenotypes

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Genes addressed in this paper
tmem104
Topics in this paper
Mammalian Gene Related
Cellular Location
Synonym
Function/Process
Cell Cycle
RNA Levels and Processing
Transcriptional Regulation X
Regulatory Role
Protein Physical Properties X
Protein Functional Domain X
Strains/Constructs
Development/Morphogenesis
Phylogenetic Analysis X
Regulated By X
Growth
Mutants/Phenotypes