dictyBase Literature
Page Contents: Abstract | Summary Chart

Robinson, N., Spudich, A., (2000) ' Dynacortin, a genetic link between equatorial contractility and global shape control discovered by library complementation of a Dictyostelium discoideum cytokinesis mutant. ' J Cell Biol 150 823-38
   Reference full text
 
Abstract:We have developed a system for performing interaction genetics in Dictyostelium discoideum that uses a cDNA library complementation/multicopy suppression strategy. Chemically mutagenized cells were screened for cytokinesis-deficient mutants and one mutant was subjected to library complementation. Isolates of four different genes were recovered as modifiers of this strain's cytokinesis defect. These include the cleavage furrow protein cortexillin I, a novel protein we named dynacortin, an ezrin-radixin-moesin-family protein, and coronin. The cortexillin I locus and transcript were found to be disrupted in the strain, identifying it as the affected gene. Dynacortin is localized partly to the cell cortex and becomes enriched in protrusive regions, a localization pattern that is similar to coronin and partly dependent on RacE. During cytokinesis, dynacortin is found in the cortex and is somewhat enriched at the poles. Furthermore, it appears to be reduced in the cleavage furrow. The genetic interactions and the cellular distributions of the proteins suggest a hypothesis for cytokinesis in which the contraction of the medial ring is a function of spatially restricted cortexillin I and myosin II and globally distributed dynacortin, coronin, and RacE.
Status: Published Type: Journal article Source: PUBMED PubMed ID: 10953006

 
Genes addressed in this paper
corA ctxA dct mhcA racE test
Topics in this paper
Genetic Interactions
X X X X
Cytoskeleton
X X X X
DNA/RNA Sequence Features

X


Cellular Location

X


Mutants/Phenotypes
X X X X
Function/Process
X X X X
Chemotaxis/Motility
X X X X


There are 2 'not yet curated' genes for this paper. corA | test |