Transformation with a particle gun

Comment 2:

The parameters listed in 7.-9. determine the speed of the particles when they hit the target (initial speed, friction and brake lenght). Our results indicate that mild bombardment is advantageous for Dictyostelium discoideum. The final parameters of 6 bar and 14 cm are at the lower limit of our apparatus. Further reduction could not be tested, but might further improve the efficiency.