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Chemoattractant receptors in chemotaxis
Confocal image of GFP-tagged chemoattractant receptors in living amoebae undergoing chemotaxis. The chemoattractant gradient is established using a micropipet located just below the frame. Images were captured every 15 seconds. From Z. Xiao and P.N. Devreotes, Johns Hopkins Medical Institutions.
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G-protein b-subunits in chemotaxis
Confocal image of GFP-tagged Gb-subunits in living amoebae undergoing chemotaxis. The chemoattractant gradient is established using a micropipet located just above the frames. Images were captured every 20 seconds. From T. Jin, N. Zhang, and P.N. Devreotes, Johns Hopkins Medical Institutions.
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PH-domain translocation with uniform stimulus
Kinetic analysis of the translocation from the cytoplasm to the plasma membrane of GFP-tagged CRAC (Cytosolic Regulator of Adenylyl Cyclase-a PH domain containing protein) in response to a uniform increase in chemoattractant concentration. Frames were taken every 2 seconds. The chemoattractant was added just before the cell goes out of focus. From C.A. Parent and P.N. Devreotes, Johns Hopkins Medical Institutions.
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PH-domain translocation during chemotaxis
Confocal image of GFP-tagged CRAC in living amoebae undergoing chemotaxis. CRAC-GFP specifically translocates to the leading edge of newly elicited pseudopods. The chemoattractant gradient is established using a micropipet located near the upper right corner of the frame. Images were captured every 5 seconds. From C.A. Parent and P.N. Devreotes, Johns Hopkins Medical Institutions.
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PH-domain translocation in immobilized cells
Translocation of CRAC-GFP in cells lacking actin filament formation. Cells chemotaxing towards the micropipet in the lower portion of the frame were treated with Latrunculin A and observed within the next few minutes. Images were captured every 5 seconds. From C.A. Parent and P.N. Devreotes, Johns Hopkins Medical Institutions.
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PH-domain dynamics in immobilized cells
Immobilized CRAC-GFP expressing cells can respond to changes in chemoattractant gradients. Latrunculin A treated cells were subjected to changing gradients of chemoattractants by moving the micropipet around the cell. Images were captured every 5 seconds. From C.A. Parent and P.N. Devreotes, Johns Hopkins Medical Institutions.
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Digitized wild-type cell in chemotaxis
Three dimensional direct image reconstruction of a D. discoideum amoebae crawling in a gradient of cAMP. The chemoattractant source is left of the field. From D. Wessel and D. Soll, Univ. of Iowa
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Digitized clathrin heavy chain null cell in chemotaxis
Three dimensional direct image reconstruction of a clathrin heavy chain null Dictyostelium cell. Note the aberrant cell shape, lack of polarity and numerous fragmented pseudopods. From D. Wessel and D. Soll, Univ. of Iowa.
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