Additional Materials

Additional Materials

The Dicty Stock Center has started to accept antibodies, cDNA and genomic libraries, and other materials people would like to share.

If you would like to send additional materials, or would like to obtain the materials listed below, please contact dictystocks@northwestern.edu.


cDNA libraries

  • Robinson expression cDNA library

    Details of the library can be found in Robinson and Spudich 2000. A 100 µl aliquot of the library in STBL2 bacteria will be shipped on dry ice or on ice. In the latter case it has to be used immediately.

Antibodies

  • Anti-calmodulin monoclonal antibodies (calA). Deposited by Margaret Clarke.
    There are several mouse monoclonal with different specificities (see paper for details): 2D1, 1F11, 4C5, 6D4, 20B9, and 15E7. Certain of those monoclonals are also availablel from Sigma and other commercial sources. Immunoblotting calmodulin requires special techniques, as described in the paper.
    Reference: Hulen et al. 1991.
  • Anti-calmodulin polyclonal antiserum (calA). Deposited by Margaret Clarke.
    These antisera (B88, B89) probably cross-react with other calmodulin-like proteins in Dictyostelium. Reference: Zhu et al. 1993.
  • Anti-cluA monoclonal antibody (cluA). Deposited by Margaret Clarke.
    The 10D5 mouse monoclonal antibodies. Reference: Zhu et al. 1997.
  • Anti-dynacortin polyclonal antiserum (dct). Deposited by Doug Robinson.
    Use at 1:10,000 to 1:50,000 dilution.
    Reference: Robinson and Spudich 2000.
  • Anti-myosin II heavy chain monoclonal antibodies (mhcA). Deposited by Margaret Clarke.
    There are four different mouse monoclonals: M342, M151, M98 and M27. M342 binds specifically to monomeric myosin in solution, presumably because the epitope is not accessible in thick filaments.
    References: Reines & Clarke 1985a, Reines & Clarke 1985b.
  • Anti-ribosomal protein L7 antibody (rpl7). Reference: Szymkowski et al. 1989.
  • Anti-discoidin-I polyclonal antiserum (dscA). Deposited by Margaret Clarke.
    Antisera from two different rabbits (Y355, Y356). Reference: Rathi and Clarke 1992.
  • Anti-vatA monoclonal antibody (vatA). Deposited by Margaret Clarke.
    The N1 and N4 mouse monoclonal antibodies were generated by Agnes Fok at the University of Hawaii. Reference: Fok et al. 1993.
  • Anti-vatM monoclonal antibody (vatM). Deposited by Margaret Clarke.
    The N2 mouse monoclonal antibodies were generated by Agnes Fok at the University of Hawaii. Reference: Fok et al. 1993.
  • Anti-dimB antibody (dimB). Deposited by Yoko Yamada, Jeff Williams
    Raised in Rabbit; use at a titre of 1/5000. References: Zhukovskaya et al. 2006 and Yamada et al. 2013
  • Anti-dimB-phosphoS5090P antibody (dimB). Deposited by Yoko Yamada, Jeff Williams
    Raised in Sheep; use at a titre of 1/300. Reference: Fukuzawa et al. 2001.
  • Anti-statc-total-7H3 antibody (dstC). Deposited by Tsuyoshi Araki, Jeff Williams
    Raised in Mouse; use at a titre of 1/500 – 1/1000. Reference: Fukuzawa et al. 2001.
  • Anti-statc-phospho-CP22 antibody (dstC). Deposited by by Tsuyoshi Araki, Jeff Williams
    Raised in Mouse; use at a titre of 1/300 – 1/500. Reference: Fukuzawa et al. 2001.

Monoclonal antibodies are also available from the Developmental Studies Hybridoma Bank where you can view the list of Antibodies against Dictyostelium discoideum.


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