About the Dicty Stock Center

About Dicty Stock Center

In the fall of 2002 the Dicty Stock Center opened at Columbia University in New York City as a repository for Dictyostelium discoideum and other cellular slime molds under the direction of Dr. Richard Kessin, and curated by Mr. Jakob Franke. The Dicty Stock Center was relocated to Northwestern University in April of 2009. As of May 2012, more than 1,850 strains can be acquired from the stock center and this number is rapidly increasing. The strains available from the stock center are in the strain catalog on our website. Additionally, as of May 2012, over 700 plasmids are available and the plasmid catalog is rapidly expanding. Several strain catalogs are listed here.

The collection is being built by requesting published strains and plasmids. We encourage and also periodically remind investigators to send new mutants, natural isolates, and plasmids, once they have been published. Validation of the materials is mostly done by observable phenotypes, while mutants are also tested for drug-resistance markers. Plasmids will be checked by performing one or two diagnostic restriction enzyme digests, and are stored both as DNA and as transformed bacteria at -80°C. However, a large component of the quality control program will consist of feedback from the recipients of materials. Strains are stored at two different locations in liquid nitrogen, either as spores or as vegetative amoebae.

The stock center is also responsible for keeping the 'Franke Dictyostelium Reference Library' up to date. This database is a nearly complete collection of almost 10,000 references specific to all aspects of the cellular slime molds, maintained in an EndNote database. The literature database can be downloaded here.


Click here for a history of the Stock Center conception.

For more procedural and technical information, see our Stock Center FAQ page.


Home| Contact dictyBase| Contact Dicty Stock Center Curators| SOPs| Site Map  Supported by NIH (NIGMS and NHGRI)