Development

Development

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Cell tracks on agaroseNEW!

At about 17 hours, individual cells lay down tracks as they crawl, at about the same time the slug begins to migrate. The experiment uses 4x phase contrast microscopy of cells developing on top of agarose and was done by Joe Molleur and Nichole Bowen in David Knecht's Cell Biology Laboratory course. Contributed by David Knecht, Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.

Early development

NC4A2 cells developing on top of an agarose sheet. The timing is given in the movie, in hours:minutes:seconds. The movie was made by Jordan Matheson and Nicholas Minotolo in Dave Knecht's Cell Biology Laboratory course. Contributed by David Knecht, Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.

Aggregation center of Crac-GFP labeled cells

Concentric waves of crac-GFP localization in an early aggregation center. Cells were developed for 10 hours on a thin layer of 1% purified agar made up in KK2. An agar block was inverted into a drop of silicone oil on a cover slip and imaged from below. From Harry MacWilliams, Ludwig-Maximilians-Universitaet Muenchen, Germany.

Chemotaxis of large field of cells

Aggregation of D. discoideum amoebae towards a point source of the chemoattractant cAMP. The numbers on the upper left corner are minutes and seconds. From G. Gerisch, Max-Planck-Institut fur Biochemie, Martinsried, Germany. 

Aggregation towards cAMP

Aggregation of D. discoideum ameobae towards cAMP, lower magnification.  From R. Firtel, University of California, San Diego

Details of Chemotaxis 

Chemotaxis of single cells towards a point source of the chemoattractant cAMP. The numbers on the upper left corner are minutes and seconds. From G. Gerisch, Max-Planck-Institut fur Biochemie, Martinsried, Germany.

Dynamic Reorganization of Actin in chemotaxing cells

Actin shows dynamic reorganization in the leading edge of cells migrating under the influence of cAMP. Actin is visualized by an actin binding domain-GFP fusion made in David Knecht's laboratory (Univ. Conn.). From R. Firtel, UCSD.

Steps cell movement in aggregation

Time-lapse video of small group of aggregating cells.  Interval between movement steps is 6 minute.  From P. Devreotes, Johns Hopkins Medical Institutions.

Dark-field waves of cell shape change in aggregation

Time-lapse video of monolayer of aggregating cells filmed through a dark-field macroscope.  White bands represent chemotactically oriented cells.  Period of wave initiation is 6 minutes.  From P. Devreotes, Johns Hopkins Medical Institutions.

Movement of Cells Into an Aggregate

Under the influence of cAMP, cells form streams which participate in the formation of a multicellular tissue by chemotaxis.  From R. Firtel, UCSD. 

Movement of Cells During Morphogenesis

Time-lapse video showing slug formation, migration and culmination.  From R. Chisholm, Northwestern University.
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