Motility
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Actin in chemotaxis
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Cells expressing GFP-actin are observed as they were chemotaxing towards a
micropipette filled with cAMP. The tip of the pipette is moved into different
positions as indicated. Images were captured every 18 seconds. From K. Barisic,
M. Ecke, C. Heizer, M. Maniak, M. Westphal, R. Albrecht, G. Gerisch, Max-Planck-Institut
fur Biochemie, Martinsried, Germany.
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Coronin in chemotaxis
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Cells expressing GFP-coronin are observed as they were chemotaxing towards a
micropipette filled with cAMP. The tip of the pipette is moved into different
positions as indicated. Images were captured every 10 seconds. From K. Barisic,
M. Ecke, C. Heizer, M. Maniak, M. Westphal, R. Albrecht, G. Gerisch, Max-Planck-Institut
fur Biochemie, Martinsried, Germany. |
Tubulin in random movement
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Cells expressing GFP-tubulin are observed during non-oriented cell migration.
Images were captured every 20 seconds. From E.L. de Hostos, UCSF, and J. Sabry, Cytokinetics, Inc., South San Francisco. |
Random Motility
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D. discoideum cells expressing GFP-tagged to a highly conserved actin
binding domain (ABD) crawling on a glass surface. DIC and fluorescence images
were collected simultaneously. Images were captured every 10 seconds.
From K.M. Pang, E. Lee, and K.A. Knecht, University of Connecticut. |
Side view of moving cell
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Side view of amoebae expressing GFP-ABD plated on a glass surface. DIC and
fluorescence images were collected simultaneously every 10 seconds by confocal
microscopy. From E. Shelden and D.A. Knecht, University of Connecticut.
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