| 370 | Δ(1-75;119-579)-golvesin(C)-GFP | Core fragment of golvesin with C-terminal GFP tag. | U. Mintert (G. Gerisch) |
| 1 | 339-3 (mRFPmars in pBsrH) | Synthetic brilliant red fluorescent protein.
The last amino acid that
belongs to mRFPmars sequence in the 339-3 vector is A. G and S correspond to the BamHI restriction site, E and F to the EcoRI restriction site. There is no His-tag encoded by 339-3 as this is made for expression in Dictyostelium. The sequence you insert into the MCS has to end with a stop codon as there is no stop present in the sequence.
| Annette Muller-Taubenberger |
| 351 | A15-aequorin | | Harry MacWilliams |
| 384 | A15-pax-gfp | Paxin rescue vector
| Gerti Weijer |
| 300 | A15-pHluorin | Cytosolic pH-sensor
| Harry MacWilliams |
| 342 | A15/CFP-Apg1 | Fusion construct of CFP and DdApg1 under the control of the actin 15 promoter (in pDXA-HC). | Grant Otto |
| 339 | A15/GFP-Apg1 | GFP-Apg1 fusion construct under the control of the actin 15 promoter (in pDXA-HC)
| Grant Otto |
| 341 | A15/GFP-Apg8 | GFP-DdApg8 fusion construct under the control of the actin 15 promoter (in pTX-GFP).
| Grant Otto |
| 330 | A15/myc-fbxa | Myc-tagged FbxA in pDXA background.
| Jakob Franke |
| 340 | A15/RFP-Apg8 | RFP-DdApg8 fusion construct under the control of the actin 15 promoter (in RFPmars). Ddatg8 was obtained by PCR with the primers GGGGATCCGTTCATGTATCAA and GGGGATCCTTATAAATCACTA and cloned into the BamHI restriction site of the plasmid RFPmars.
| Grant Otto |
| 279 | aar-KO vector | aardvark knockout vector
| Adrian Harwood |
| 42 | ACA | Partial ACA cDNA (adenylyl cyclase) in pBlue Script.
| Peter Devreotes |
| 81 | acbA-bsr | acbA knockout vector | Bill Loomis |
| 334 | ACG KO Hygro | | Pauline Schaap |
| 385 | ACG-KO | Knockout plasmid for acgA
| Karin Weening (Pauline Schaap) |
| 314 | Act 15 ACG | | Pauline Schaap |
| 322 | Act 15 ACG-Y | | Pauline Schaap |
| 107 | amiB-KO (p12-3NdeI) | Recapitulation of the R12-3 amiB- REMI mutant (aggregation-minus B)
| Yukako Asano (Kazuo Sutoh) |
| 106 | amiB-KO (p82ClaI) | Recapitulation of the R8-2 amiB- REMI mutant (aggregation-minus B)
| Yukako Asano (Kazuo Sutoh) |
| 460 | arpB-KO | Knockout construct for arpB (blasticidin cassette from pRHI148 was excised as a ClaI fragment and ligated into the BstBI site of arpB) | |
| 202 | atgN-KO | atgN knockout construct containing bsr cassette.
| Turgay Tekinay |
| 321 | BS15 | | Pauline Schaap |
| 14 | calA | calA cDNA in PET15b (Dicty calmodulin I)
| Barrie Coukell |
| 17 | calnexin-GFP | Calnexin-S65T-GFP in pDEXRH(G418)
| Annette Muller-Taubenberger |
| 306 | calreticulin pHluorin | ER pH-sensor | Harry MacWilliams |
| 124 | carB::lacZ | carB-lacZ expression construct
| Karl Saxe |
| 56 | cbpA-KO | cbpA (calcium-binding protein 1)
gene disruption construct. | Barrie Coukell |
| 15 | cbpJ | cbpJ cDNA in PET15b (CbpJ protein) calcium-binding protein
| Barrie Coukell |
| 68 | CRAC-GFP (pWf1) | Full-length cDNA of CRAC was cloned upstream of the start codon of GFP. The resulting CRAC-GFP construct was inserted in the constitutive expression vector B18.
| Peter Devreotes |
| 158 | CS4 5.03 | Blasticidin cassette is loxed; plasmid can be used to replace the endogenous NSFA gene with the ts variant, which exists in HM1067. Plasmid is similar to the unloxed version used to generate NSFA2 (Pmid 12183371; d7379).
| Mark Bretscher |
| 57 | cupB-AS | cupB (Ca2+/Calcineurin-regulated cup gene) antisense construct.
| Barrie Coukell |
| 299 | dimA-KO | dimA knockout construct | Chris Thompson |
| 315 | dimB-KO | dimB knockout vector.
| Gad Shaulsky |
| 230 | DIV1 | remi plasmid (not yet available)
| Bill Loomis |
| 228 | DIV2 | Remi plasmid (DIV2 is the same as DIV1 shown below, except it contains the pyr5-6-containing ClaI fragment in the opposite orientation).
| Bill Loomis |
| 239 | DIV5 | remi plasmid for uracil selection (not yet available) | Bill Loomis |
| 229 | DIV6 | Remi plasmid. The PstI-SacI pyr5-6 fragment from DIV1 was ligated into PstI/SacI digested pGEM-5Zf(+) (Promega).
| Bill Loomis |
| 89 | dng-KI | Dominant-negative knock-in construct for Gγ (Dicty Gγ without the C-terminal four amino acids). Same as GγΔ?
| Peter Devreotes |
| 259 | DNG1-GFP | DNG1-GFP vector | Yasuo Maeda |
| 257 | DNG1-KO | DNG1 knockout vector
| Yasuo Maeda |
| 258 | DNG1-OE | DNG1 overexpression vector
| Yasuo Maeda |
| 280 | dpoA KO construct | dpoA knockout vector (plasmid rescued with ClaI from REMI mutant HAD172).
| Adrian Harwood |
| 492 | DsRed | DsRed expression vector pDG75 transformed into E. coli XL-1. Expresses red bacteria when grown in LB with ampicillin (75 µg/ml) and 1 mM IPTG.
| David Knecht |
| 46 | EcmA-Gal | EcmA PCR'd promoter fragments inserted into BamHI site of A15deltaBam-gal. 3'end, Bam/BglII at +250 (just upstream of EcmA-ATG). 5'end, BamHI site at variable points. (prestalk marker)
| Jeff Williams |
| 344 | ecmA/myc-fbxA | Myc-tagged FbxA under the control of the prestalk ecmA promoter. | Jakob Franke |
| 50 | EcmAO-Gal | Galactosidase fusion expression vector (prestalk marker)
| Jeff Williams |
| 51 | EcmB-Gal | Galactosidase fusion expression vector (prestalk marker)
| Jeff Williams |
| 47 | EcmO-Gal | EcmA PCR'd promoter fragments inserted into BamHI site of A15deltaBam-gal. 5' end, Bam/BglII at -1694. 5' end, BamHI site at variable points.
(5' junction of available promoter)
| Jeff Williams |
| 356 | EXP4(+) | Integrating expression vector based on pATSP.
| Karin Weening (Pauline Schaap) |
| 357 | EXP5(+) | Integrating expression vector derived from EXP4(+) by removal of the HindIII and XbaI sites, and replacement of the mcs.
| Karin Weening (Pauline Schaap) |
| 55 | EXPpatA-AS | patB (Ca2+-ATPase PAT1) antisense construct | Barrie Coukell |
| 10 | EZTN:tetR-bsR | Vector containing a transposable bsR cassette.
| Tomo Abe |
| 104 | FadA-KO | fadA knock-out vector (Bsr cassette inserted in the HindIII site)
| Tamao Saito |
| 105 | FadB-KO | fadB knockout vector with the Bsr cassette inserted in the BglII site.
| Tamao Saito |
| 346 | fbxA/gfp-neo | Plasmid that expresses GFP under the control of the fbxA promoter (in a pTX-GFP background). | Jakob Franke |
| 332 | fbxA/myc-fbxA | Myc-tagged FbxA under the control of the fbxA promoter in a pDXA background.
| Jakob Franke |
| 302 | Gα2-CFP | Reporter construct (CFP inserted into the helical domain of Gα2 in a location optimal for FRET).
| Jane Borleis (Devreotes) |
| 99 | Gβ-YFP | Reporter construct (YFP fused to NH2-terminal of Gβ). The gene encoding the full-length YFP was fused to the NH2-terminus of the Gβ gene. A BglII restriction site was added at the junction that consisted of two additional amino acids, arginine and serine. This β-YFP fusion protein was cloned into the CV5 vector. CV5 was derived from p88 by addition of an actin 15 expression cassette from pMC34.
| Tian Jin |
| 64 | Gγ-YFP | Gγ-YFP construct (unpublished) | Peter Devreotes |
| 371 | GFP-(N)-Δ(1-75)-golvesinGFP | Golvesin lacking the N-terminal 75 amino acids with C-terminal GFP tag. | U. Mintert (G. Gerisch) |
| 373 | GFP-(N)-Δ(559-579)-golvesin | Golvesin with C-terminal deletion and N-terminal GFP tag. | U. Mintert (G. Gerisch) |
| 374 | GFP-(N)-golvesin-(L9A,L10A) | Full-length golvesin lacking the dileucine motif, with N-terminal GFP tag.
| U. Mintert (G. Gerisch) |
| 108 | GFP-Arp2/pBIG(G418) | The arp2 fragment was subloned into the pBIG plasmid downstream of the actin15 promoter, the GFP coding sequence, and a Gly-Pro-Gly linker sequence, and upstream of the actin15 terminator.
| Yukako Asano (Taro Uyeda) |
| 18 | GFP-calreticulin | S65T-GFP-calreticulin in pDEXRH(G418)
| Annette Muller-Taubenberger |
| 98 | GFP-Gβ | Reporter construct (GFP fused to NH2-terminus of Gβ subunit). The coding region of GFP-Gβ fusion protein was cloned into pMC34, an extrachromosomal expression vector.
| Tian Jin |
| 369 | Golvesin(C)-GFP | Golvesin with C-terminal GFP tag
| U. Mintert (G. Gerisch) |
| 372 | Golvesin(N)-GFP | Golvesin with N-terminal GFP tag.
| U. Mintert (G. Gerisch) |
| 223 | gp130-ura | knockout plasmid for gp130 (uracil cassette in genomic gp130) | Cathy Chia |
| 442 | iplA(ins) | iplA knockout vector. | David Lam (Pierre Golstein) |
| 405 | K-neo | Dd PK2 protein kinase over-expression vector.
| Christophe Anjard |
| 406 | Kdel-neo | Mutated Dd PK2 protein kinase over-expression vector. (K-neo cut with KpnI, blunt-ended with T4-polymerase, and religated)
| |
| 316 | lim2- | Kanamycin-resistant limB-knockout vector (in pCR-BluntII-TOPO) with hygromycin-resistance cassette.
| Gerti Weijer |
| 246 | LST8-KO | LST8 knockout vector | Rick Firtel |
| 247 | LST8-OE | LST8 overexpression vector | Rick Firtel |
| 292 | lvsB-KO | Knockout vector with bsR cassette for lvsB gene
| Arturo De Lozanne |
| 293 | lvsC-KO | Knockout vector with bsR cassette for lvsC gene
| Arturo De Lozanne |
| 294 | lvsD-KO | Knockout vector with bsR cassette for lvsD gene
| Arturo De Lozanne |
| 295 | lvsE-KO | Knockout vector with bsR cassette for lvsE gene
| Arturo De Lozanne |
| 296 | lvsF-KO | Knockout vector with bsR cassette for lvsF gene
| Arturo De Lozanne |
| 474 | mars-ABD120 | Vector with a bsR-resistance cassette, expressing a fusion of the actin-binding domain of ABD120 (filamin) and RFP.
The plasmid was originally constructed by Annette Mueller-Taubenberger. The sequence was repaired by Dave Knecht (Y218N)
| David Knecht (Annette Mueller-Taubenberger) |
| 475 | mars-LIMΔcoil | Vector containing a bsR-resistance cassette, expressing a fusion of RFPmars and LIMΔcoil.
The plasmid was constructed by Annette Mueller-Taubenberger | David Knecht (Annette Mueller-Taubenberger) |
| 264 | MB35-M2 (tetON driver) | Both tetON and tetOFF systems exist for Dicty. The tetOFF system was pioneered by Dingermann and colleagues, but the most frequently used
vectors are those constructed by Blaauw et al., Gene 252:71-82, 2000.
The Dicty tetON system is derived from yeast vectors described in Urlinger
et al., PNAS 97:7963-8, 2000; the rtTa-M2 element from ? was recloned into the Blaauw vector MB35 by Adriano Ceccarelli, and students in my lab have shown that the performance of this tetON system in Dicty (induction
factor, response time) is similar to that of the tetOFF system.
Both the original tetOFF MB35 and its tetON version are neo vectors. In practice one needs a Dicty strain carrying one or the other of these, which one overtransforms with a second, blasticidin vector (Blauuw et al. MB38) carrying the gene of interest. Once one has cloned one's gene into MB38, it can be overtransformed into both tetON and tetOFF strains. Both MB35 and MB38 are in the stock center, as are cells containing MB35. The tetON version of MB35 will go to the stock center today.
| Harry MacWilliams |
| 367 | MF1 | cudA knockout vector obtained from original REMI mutant (using pRHI30) by EcoRI digestion and religation. | Masashi Fukusawa |
| 368 | MF2 | cudA knockout vector containing an internal deletion and bsr cassette.
| Masashi Fukusawa |
| 462 | mrrA:lacZ | Complete mrrA upstream promoter region fused to lacZ. | Susan Ross (Jeff Williams) |
| 317 | Myc10 | | Pauline Schaap |
| 13 | ncsA | ncsA cDNA in PET15b (NcsA protein)
| Barrie Coukell |
| 16 | ncsA-KO vector | ncsA cDNA clone (SSL268) in pBluescript KS - with the blasticidin S cassette from pBsR519 cloned into the ClaI site near the middle of the coding region.
| Barrie Coukell |
| 365 | Nramp1-KO vector | Nramp1 knockout vector | Salvo Bozzaro |
| 200 | p | | Dietmar Manstein |
| 224 | p155d1 | 7268-bp Csp451 fragment of Ddp1 in p60.
| Carole Parent |
| 218 | p240Cla | erkB knockout vector (2.1-kb and 1.7-kb genomic fragments flanking the remi plasmid DIV6) | Bill Loomis |
| 225 | p60 | Dictyostelium expression vector: 2.3-kb SalI-EcoRI fragment (neoR cassette) of pB10S subcloned into pGEM3Z.
| |
| 43 | p6B (CAR1) | Partial cAR1 cDNA in pBlue Script.
| Peter Devreotes |
| 86 | pA15 | | Peter Devreotes |
| 191 | pA15-PsiA(ORF) | psiA rescue vector, in Exp4(+), under the control of the actin 15 promoter. | Takefumi Kawata |
| 48 | pA15-Rm | (pActRsub-AEBE)
Two glycine->glutamic acid mutations in the regulatory subunit of PKA. Overexpression results in inhibition of the catalytic subunit in the presence of cAMP.
| Jeff Williams |
| 117 | pA15Gal | Galactosidase expression vector with actin 15 promoter.
| David Knecht |
| 311 | pA15lagC | | |
| 468 | pA15TX | | |
| 469 | pA15XPT1 | | |
| 470 | pA6NPTII | | |
| 461 | pACA-KO | Two DNA fragments were amplified using genomic DNA as template. One of the fragments corresponds to the region upstream of the aca open reading frame. The other one corresponds to the region encoding the middle part of the aca open reading frame. The blasticidin cassette was also amplified by PCR and inserted in the middle of both cyclase fragments using the metod of Kuwayama et al. (Pmid 11788728; d7349), and cloned into pCR-BluntII-TOPO. | Satomi Matsuoka (M. Ueda) |
| 87 | pACA.URA | ACA knockout construct
| Peter Devreotes |
| 271 | pActin15-GskA | gskA expression vector (actin15::gskA) made by subcloning the gskA cDNA into pDXA-3H (gskA contains C-terminal 6xHistidine tag).
| Adrian Harwood |
| 274 | pActin15-Rc | actin15::PKA-Rsubunit (Rc mutant does bind C subunit = control) expression vector.
| Adrian Harwood |
| 273 | pActin15-Rm | actin15::PKA-Rsubunit (Rm mutant) expression vector.
| Adrian Harwood |
| 272 | pActin15-Rsub1 | actin15::PKA-Rsubunit (wild type) expression vector containing the actin6-neoR cassette.
| Adrian Harwood |
| 265 | pActin15Gal | Galactosidase vector
| Adrian Harwood |
| 283 | pActin15StatAcidpep | actin15::StatA containing Ser-Asp GSK3 peptide specific substrate
| Adrian Harwood |
| 282 | pActin15StatAlapep | actin15::StatA containing Ser-Ala GSK3 peptide specific substrate
| Adrian Harwood |
| 281 | pActin15StatWTpep | actin15::StatA containing WT GSK3 peptide specific substrate
| Adrian Harwood |
| 59 | pAK2 | carB-KO construct (car2-::thy)
| Karl Saxe |
| 221 | pAmpA/lacZ | ampA promoter-lacZ fusion construct | Daphne Blumberg |
| 62 | pATψ-BsrR-Rev | Targeting vector for the psiA gene | Takefumi Kawata |
| 54 | patB-KO | patB (P-type H+-ATPase) gene disruption construct. | Barrie Coukell |
| 312 | pax1- | pax1 knockout vector (in pBLuescript-SKII) containing bsr cassette.
| Gerti Weijer |
| 226 | pB10S | Not available yet. Dictyostelium expression vector containing neoR cassette (actin 6 promoter, Tn5 neomycin resistance gene, actin 8 terminator (in reverse)). | |
| 291 | pB10TP4 | Not available.
Same as pB10TP2 except for a modification in the polylinker (JG Williams, pers. commun.) | |
| 25 | pB18 | Integrating expression vector. containing the actin6 neo cassette.
| Peter Devreotes |
| 487 | pBC18 | Chimeric protein, with the extracellular portion of the contact site A protein fused to the transmembrane domain of the integral protein P29F8 and to the cytoplasmic sequence KTRVSQNSG, in the expression vector pDCEV4. | Francois Letourneur |
| 121 | pBIG | Autonomous replicating extrachromosomal expression vector with G418 castte. | Tom Egelhoff |
| 381 | pBIG-GFP-myo | Expresses mhcA with GFP fused at N-terminus, driven by actin 15 promoter. Use G418 selection for Dicty, ampicillin for bacteria. | Tom Egelhoff |
| 379 | pBIGALA | Expresses mhcA fused to actin 15 promoter, carrying "3xALA" mutation of threonines 1823, 1833, and 2029. Use G418 selection for Dicty, ampicillin for bacteria. | Tom Egelhoff |
| 380 | pBIGASP | Expresses mhcA fused to actin 15 promoter, carrying "3xASP" mutation of threonines 1823, 1833, and 2029. Use G418 selection for Dicty, ampicillin for bacteria. | Tom Egelhoff |
| 386 | pBORP | Expression vector containing neoR cassette.
| Rex Chisholm |
| 389 | pBS18/car2 | | Karl Saxe |
| 85 | pBSA15 | A15-neo cassette cloned in pBluescript in its BamHI-EcoRI site.
| Peter Devreotes |
| 407 | pBSEGE | The plasmid pBSEGE was rescued from egeA- genomic DNA by circularization of a HindIII fragment. | |
| 471 | pBSR-GFPABD120 | Vector containing a blasticidin resistance cassette, expressing a fusion of GFP and the actin-binding domain of ABD120 (filamin).
| David Knecht |
| 208 | pBsr-Nsi-MHCKD-KO | mhkD knockout vector
| Tom Egelhoff |
| 32 | pBSR1 | REMI plasmid with blasticidin cassette, with the sequence CAACAAGATCTAGAATTAG changed to CAACAAGATCCAGAATTAG to eliminate the internal BglII and XbaI sites to make it more convenient for REMI usage.
| Gadi Shaulsky |
| 33 | pBSR3 | REMI plasmid containing blasticidin cassette (SmaI and ApaI sites were introduced in pBSR1 flanking the BamHI site)
| Gadi Shaulsky |
| 37 | pBsR479 | ampicillin-resistant vector with bsR cassette
| Frantisek Puta |
| 38 | pBsR503 | Kanamycin-resistant vector with bsR cassette
| Frantisek Puta |
| 36 | pBsR519 | ampicillin-resistant vector with bsR cassette
| Frantisek Puta |
| 414 | pBsrH | Expression vector derived from pDEXH by replacing the neoR cassette with the bsR cassette.
| Markus Maniak |
| 70 | pCAR1-B18 | cAR1 over-expression vector
| Peter Devreotes |
| 93 | pCAR1-GFP | | Jane Borleis (Peter Devreotes) |
| 28 | pCAR2-B18 | cAR2 over-expression vector
| Peter Devreotes |
| 29 | pCAR3-B18 | cAR3 over-expression vector
| Peter Devreotes |
| 73 | pCAR3-KO-ura | Not sure whether it is the 5cAR3ura vector described in Johnson et al. (1993) (d4673) | Peter Devreotes |
| 34 | pCFC5 | Expression vector containing N-terminal epitope tag (T7 antigen)
| Gene Katz |
| 35 | pCFC6 | Expression vector containing N-terminal epitope tag (T7 antigen)
| Jakob Franke |
| 352 | pCotB-LacZ | Reporter construct expressing β-galactosidase under control of the prespore cotB promoter. | Bill Loomis |
| 24 | pCP33 | Extrachromosomal vector with neo cassette. Based on p155d1, with multiple cloning site added.
| Peter Devreotes |
| 227 | pCP43 | ACA (adenylyl cyclase) expression vector: ACA cDNA with act15 promoter cloned into ApaI/BstXI sites of pCP43. The ACA cDNA came with its own terminator (800-bp 3'-untranslated region)
| Carole Parent |
| 232 | pCT7 | dmtA promoter driving gfp
| Rob Kay |
| 23 | pCV5 | CV5 is derived from p88 by the addition of an actin 15 expression cassette from pMC34.
| Peter Devreotes |
| 270 | pD19-lacZ | LacZ (β-galactosidase) expression under control of the prespore promoter D19 (Psa or pspA).
| Adrian Harwood |
| 185 | pDd31P4 Neo | spiA promoter in pDdGal16 | Del Richardson (via Stefan Pukatzki) |
| 39 | pDd56 | EcmB (ST310) cDNA clone isolated as a prestalk-enriched, DIF-inducible mRNA (2.4-kb cDNA cloned in the PstI site of pUC8). DDB0216219
| Jeff Williams |
| 40 | pDd63 | EcmA (ST430) cDNA clone isolated as a prestalk-enriched, DIF-inducible mRNA (2.3-kb cDNA cloned in the PstI site of pUC8); DDB0220137
| Jeff Williams |
| 266 | pDdGal-15 (H+) | Galactosidase vector with HindIII site after terminator.
| Adrian Harwood |
| 267 | pDdGal-16 (H-) | Galactosidase vector without HindIII site after terminator.
| Adrian Harwood |
| 268 | pDdGal-17 (H+) | Galactosidase vector with HindIII site after terminator.
| Adrian Harwood |
| 269 | pDdGal-17 (H-) | Galactosidase vector without HindIII site after terminator.
| Adrian Harwood |
| 217 | pDE-0.9 | Coding region of the extracellular phosphodiesterase. (0.9-kb BstBI-EcoRI fragment, from cDNA clone p-1.2, subcloned in pUC19 digested with AccI and EcoRI)
| Jakob Franke |
| 119 | pDE102 | Vector containing hygromycin cassette
| Tom Egelhoff |
| 187 | pDE104 | | Tom Egelhoff |
| 120 | pDE109 | Non-autonomous extrachromosomal expression vector with hygromycin selection cassette.
| Tom Egelhoff |
| 8 | pDEX-NLS-cre | Dictyostelium Cre expression vector for the generation of multiple gene disruptions
| Jan Faix |
| 409 | pDEXH | Expression vector based on pIC20R. Expression of inserted cDNAs is driven by an actin 15 promoter and terminated by an actin 8 tandem terminator. Bacterial Tn5 phosphotransferase under the control of the actin 6 promoter allows selection of Dictyostelium transformants with G418. | Jan Faix |
| 366 | pDEXH-GFP-PiaAHSB1 | GFP-PIA expression vector (temperature sensitive)
| Salvo Bozzaro |
| 290 | pDEXH-GFP-PiaAWT | GFP-Pianissimo expression vector. piaAWT was subcloned into the pDEXH vector containing the GFP gene.
| Salvatore Bozzaro |
| 410 | pDEXRH | Modified version of pDEXH. Expression vector based on pIC20R. Expression of inserted cDNAs is driven by an actin 15 promoter and terminated by an actin 8 tandem terminator. Bacterial Tn5 under the control of the actin 6 promoter allows selection of Dictyostelium transformants with G418.
| Jan Faix |
| 101 | pDF15 | myosin heavy chain kinase gene replacement vector containing the Thy1 gene
| Tom Egelhoff |
| 473 | pDH-GFPABD120 | Vector containing a hygromycin-resistance cassette expressing a fusion of GFP and the actin-binding domain of ABD120 (filamin).
| David Knecht |
| 355 | pDH29 | Remi plasmid for ura selection (derived from pJB1)
| Dale Hereld |
| 113 | pDNeo2-Dd-TRAP1 | Dd-TRAP1 overexpression vector
| Yasuo Maeda |
| 74 | pDNeo67 | Expression vector derived from pDNeoII by BAL31 digestion of the 4 methionine codons between the actin6 promoter and the mcs. The start codon has to be provided by the insert.
| C. Klein? |
| 479 | pDNeo67-dia2-gfp | Vector expressing DIA2-GFP. | Aiko Amagai |
| 103 | pDNeoII (pDNeo2) | Expression vector including the start codon and first 8 amino acids of actin 6.
| A. Noegel? |
| 464 | pDRH:GFP-tubulin | Ddp1-based expression vector for GFP-tubulin containing a hygromycin selection cassette. | Douglas Robinson |
| 465 | pDRH:RFP-tubulin | Ddp1-based expression vector for RFP-tubulin containing a hygromycin selection cassette. | Douglas Robinson |
| 463 | pDRHyg | Ddp1-based expression vector containing a hygromycin selection cassette. | Douglas Robinson |
| 320 | pdsB KO1234 | | Pauline Schaap |
| 319 | pdsB KO5234 | | Pauline Schaap |
| 233 | pDT1 | regA KO bsr. Insert blasticidin cassette into AHindIII332pKS (pDT4) as Xba fragment, then insert 3' homology as NotI/SacII PCR products.
| Rob Kay |
| 235 | pDT12 | regA driven by actin 15
| Rob Kay |
| 234 | pDT5 (iplA-KO2) | iplA knockout construct
| Rob Kay |
| 301 | pDU3B1 | DdPYR5-6 gene (3.7-kb genomic DNA in pBR322)
| Jakob Franke |
| 325 | pDV-CGFP | Send request to Pauline Schaap. Tandem affinity purification vector with expression under control of the actin 15 promoter, and a C-terminal GFP tag.
| Pauline Schaap |
| 326 | pDV-CGFP-CTAP | Tandem affinity purification vector with expression under control of the actin 15 promoter, and a C-terminal GFP and C-terminal TAP
| Pauline Schaap |
| 243 | pDV-CTAP | Tandem affinity purification vector with expression under control of the actin 15 promoter, and a TAP tag at the C-terminus.
| Pauline Schaap |
| 313 | pDV-CYFP | Send request to Pauline Schaap. Tandem affinity purification vector with expression under control of the actin 15 promoter, and a YFP tag at the C-terminus.
| Pauline Schaap |
| 329 | pDV-CYFP-CTAP | Tandem affinity purification vector with expression under control of the actin 15 promoter, and a C-terminal YFP and C-terminal TAP tag.
| Pauline Schaap |
| 244 | pDV-NTAP | Tandem affinity purification vector with expression under control of the actin 15 promoter, and a TAP tag at the N-terminus.
| Pauline Schaap |
| 333 | pDV-NTAP-CGFP | Tandem affinity purification vector with expression under control of the actin 15 promoter, and an N-terminal TAP tag and C-terminal GFP.
| Pauline Schaap |
| 335 | pDV-NTAP-CYFP | Tandem affinity purification vector with expression under control of the actin 15 promoter, and an N-terminal TAP tag and C-terminal YFP
| Pauline Schaap |
| 331 | pDV-NTAP-NYFP | Tandem affinity purification vector with expression under control of the actin 15 promoter, and N-terminal TAP and YFP tags.
| Pauline Schaap |
| 327 | pDV-NYFP | Tandem affinity purification vector with expression under control of the actin 15 promoter, and an N-terminal YFP tag.
| Pauline Schaap |
| 328 | pDV-NYFP-CTAP | Tandem affinity purification vector with expression under control of the actin 15 promoter, and an N-terminal YFP and C-terminal TAP tag.
| Pauline Schaap |
| 63 | pDXA-3C | Expression vector under control of the actin 15 promoter and carrying a C-terminal c-myc tag.
| Dietmar Manstein |
| 199 | pDXA-3FLAG | Dictyostelium expression vector with an N-terminal FLAG tag.
| Dietmar Manstein |
| 198 | pDXA-3H | Expression vector under control of the actin 15 promoter and carrying a C-terminal histidine tag. | Dietmar Manstein |
| 336 | pDXA-3H-BL | pDXA-3H in which the G418-resistance cassette has been replaced with a blasticidin-resistance marker.
| Doug Robinson |
| 186 | pDXA-3H-Hygro | Dictyostelium expression vector derived from the vector pDXA-3H (6.1 kb), containing an hygromycin resistance cassette and a C-terminal histidine tag. | Dietmar Manstein |
| 95 | pDXA-CFP-MCS | Dictyostelium expression vector with an N-terminal CFP tag.
| Dietmar Manstein |
| 109 | pDXA-FLAG | Dictyostelium expression vector based on Ddp2 (extrachromosomal in the presence of pREP)
| Tom Egelhoff |
| 110 | pDXA-GFP2 | Dictyostelium expression vector based on Ddp2 (extrachromosomal in the presence of pREP)
| Tom Egelhoff |
| 472 | pDXA-GFPABD120 | Vector expressing a fusion protein between GFP and the actin-binding domain of ABD120 (filamin).
| David Knecht |
| 203 | pDXA-GST | Dictyostelium expression vector with an N-terminal GST tag and a C-terminal histidine tag. | Dietmar Manstein |
| 31 | pDXA-HC | Expression vector under control of the actin 15 promoter and carrying an N-terminal histidine tag and a C-terminal c-myc tag.
Plasmid has been resequenced.
| Dietmar Manstein |
| 96 | pDXA-HY | Dictyostelium expression vector with proteins expressed from the actin 15 promoter and providing an N-terminal 7-His tag and a C-terminal YL1/2-epitope tag.
| Dietmar Manstein |
| 94 | pDXA-MCS-YFP | Dictyostelium expression vector with a C-terminal YFP tag.
| Dietmar Manstein |
| 201 | pDXA-YFP-MCS | Dictyostelium expression vector with an N-terminal YFP tag.
| Dietmar Manstein |
| 263 | pDXA/VatM-GFP | VatM-GFP fusion protein expression vector
| Margaret Clarke |
| 204 | pDXD-3C | Dictyostelium expression vector under control of the inducible discoidin Iγ promoter and carrying a C-terminal c-myc tag.
| Dietmar Manstein |
| 205 | pDXD-3H | Dictyostelium expression vector under control of the inducible discoidin Iγ promoter and carrying a C-terminal histidine tag. | Dietmar Manstein |
| 277 | pEcmA-Rc | EcmA::PKA-Rsubunit (Rc mutant does bind C subunit = control) expression vector
| Adrian Harwood |
| 278 | pEcmA-Rm | EcmA::PKA-Rsubunit (Rm mutant) expression vector
| Adrian Harwood |
| 90 | pEgeA-GFP | GFP fused to the COOH-terminal of EgeA expression vector. | Peter Devreotes |
| 486 | pFL474 | apm2-knockout construct. PCR-amplified 3' and 5' regions of apm2 (mu2 or ?2), with a bsR cassette inserted in between, in pBluescript.
| Francois Letourneur |
| 488 | pFL505 | Full-length apm1 (mu1 or µ1) expression construct in pDXA-3C.
| Francois Letourneur |
| 484 | pFL603 | apm1-knockout construct. PCR amplified 3'- and 5'-regions of apm1 (mu1 or ?1), with bsR cassette inserted in between, in pBluescript.
| Francois Letourneur |
| 489 | pFL619 | γ-Adaptin-GFP expression construct in pDXA-3C. | Francois Letourneur |
| 491 | pFL674 | CsA-Rh50
| Francois Letourneur |
| 490 | pFL712 | Full-length cDNA sequence coding for Phg2 was cloned into the pDXA-GFP2 expression vector.
| Francois Letourneur |
| 242 | pflCAP-GFP | Full-length CAP cloned in frame with GFP in pDEX-T65S-GFP.
| Angelika Noegel |
| 219 | pG3Neo-AmpA | Construct used to generate Ampa overexpressors and to rescue AmpA knockouts
| Daphne Blumberg |
| 231 | pG3NeoI | Expression vector in which the expression of the neomycin phosphotransferase gene (Tn903) is driven by the actin 15 promoter.
| Bill Loomis |
| 80 | pGACG | Insertion of fragment from pλ22 into pG1D at the common XbaI site. | Peter Devreotes |
| 284 | pGEG02-K | Rho GTPase (RacF2) knockout construct for KAX3
| Tetsuya Muramoto (Hideko Ursushihara) |
| 285 | pGEG02-V | Rho GTPase (RacF2) knockout construct for V12
| Tetsuya Muramoto (Hideko Ursushihara) |
| 193 | pGEG04-KAX3 | | Hideko Urushihara |
| 192 | pGEG04-V12 | | Hideko Urushihara |
| 190 | pGEG10 | | Hideko Urushihara |
| 382 | pGFP-MHC-3xALA | Expresses "3xALA" mutant version of Dictyostelium mhcA with GFP fused at N-terminus, driven by actin 15 promoter. Use G418 selection for Dicty, ampicillin for bacteria.
| Tom Egelhoff |
| 383 | pGFP-MHC-3xASP | Expresses "3xASP" mutant version of Dictyostelium mhcA with GFP fused at N-terminus, driven by actin 15 promoter. Use G418 selection for Dicty, ampicillin for bacteria. | Tom Egelhoff |
| 493 | pGMET-Easy-srfB-bsr | Knockout plasmid for srfB using blasticidin selection. | Leandro Sastre |
| 79 | pGSP1 | Extrachromosomal ACG expression vector (coding sequence of ACG inserted in pJK1).
| Peter Devreotes |
| 452 | pGSP10 | ACA fragment from pGSP9 inserted into the Dictyostelium extrachromosomal expression vector pATANB43. pGSP10 was used to generate aca-/ACA cells.
| Carole Parent |
| 91 | pGSP2 | Plasmid containing genomic fragment of ACA.
| Peter Devreotes |
| 449 | pGSP6 | | Jane Borleis (Devreotes) |
| 451 | pGSP9 | Full-length genomic construct of ACA containing about 1 kb of 5-prime untranslated sequence.
| Carole Parent |
| 69 | PH-GFP (pWf38) | 700-bp N-terminal PH domain of CRAC fused to GFP, by cloning it into the GFP expression vector pb15rsGFP. This gives constitutive expression under the control of the actin 15 promoter. | Peter Devreotes |
| 453 | pHygTm(plus)/pG7 | Vector containing new hygromycin Bam cassette with cabA terminator. EcoR1 and BglII within Hyg were killed by mutagenesis. Made by Masashi Fukusawa (pers. commun. Jeff Williams, 5-8-08)
| Susan Ross (Jeff Williams) |
| 305 | pi3k2-gfp | Reporter construct: pi3k2 ligated to GFP and expressed in EXP-4(+) | Jane Borleis |
| 375 | piaA-HSB1 | Mutated form of the piaA gene, containing the temperature-sensitive mutation, isolated from the mutant HSB1 and inserted into pBlueScript.
| Salvo Bozzaro |
| 289 | piaAWT | Pianissimo expression vector used to rescue the HSB1 strain. Insert is most likely in pMyc86/6, but could also be in pBluescript.
| Salvatore Bozzaro |
| 349 | pJB1 | pyr5-6 gene (ClaI fragment of pDU3B1) in pBluescript.
| Jane Borleis (Devreotes) |
| 350 | pJB22 | gufB (Gene of Unknown Function) | J. Franke/R. Kessin |
| 188 | pJH138 | Gα4-KO construct (Gα4::pyr5-6)
| Jeff Hadwiger |
| 125 | pJH154 | BamHI genomic fragment (4.2 kb) containing the entire Gα4 gene (including promoter, terminator, etc.) cloned into BamHI site of pT3T718U (Pharmacia). A 2.2-kb EcoRI-BamHI neoR cassette from B10SX was inserted into EcoRI site. | Jeff Hadwiger |
| 126 | pJH161 | Gα4::lacZ fusion containing the promoter and first 17 codons of Gα4 gene. Inserted into pAT153L cloning vector. Also contains a neoR cassette (EcoRI fragment). | Jeff Hadwiger |
| 127 | pJH206 | XhoI-BclI genomic fragment (3.1 kb) containing entire Gα5 gene (including promoter, terminator, etc.) cloned into pT3T718U with added BclI site (Pharmacia). A 2.2kb EcoRI-BamHI neoR cassette from B10SX was inserted into EcoRI site. | Jeff Hadwiger |
| 128 | pJH210 | Gα5::lacZ fusion containing the promoter and first 14 codons of Gα5 gene. Inserted into pAT153L cloning vector. Also contains a neoR cassette (EcoRI fragment). | Jeff Hadwiger |
| 129 | pJH214 | PCR segment of the Gα5 gene that was later disrupted with the Thy1 gene to yield vector pJH216.
| Jeff Hadwiger |
| 454 | pJH216 | Gα5 gene knockout construct (gα5::Thy1). Thy1 gene replaces a PstI-SpeI fragment in Gα5 genomic fragment. Excise gene disruption fragment with EcoRV-EcoRI digest.
| Jeff Hadwiger |
| 456 | pJH280 | HindIII/KpnI frag from pUCBsrBam inserted into the same sites of pBluescript SK- (Stratagene). Many restriction sites available for excising Bsr gene. Reference for pUCBsrBam - Adachi H, Hasebe T, Yoshinaga K, Ohta T, Sutoh K. (1994) Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the blasticidin S resistance marker. Biochem Biophys Res Commun. 205:1808-14. | Jeff Hadwiger |
| 455 | pJH48 | Pyr5-6 gene with SalI linker inserted at PvuII site, can be excised with Asp718 and used to create pyr5-6- mutants (e.g., JH8)
| Jeff Hadwiger |
| 21 | pJK1 | Extrachromosal vector containing a neoR cassette derived from the Ddp1-based vector pATANB43 (Pmid 2813371) into which the actin15 promoter and the 2H3 terminator have been inserted.
| Peter Devreotes |
| 22 | pJK3 | cAR1 cDNA cloned in the extrachromosomal expression vector pJK1 | Peter Devreotes |
| 388 | pJR1 | | Karl Saxe |
| 445 | pJRC119 | GFP-Set1 rescue construct (in pDEXH82).
| Jonathan Chubb |
| 444 | pJRC13 | Set1 bsr disruption plasmid
| Jonathan Chubb |
| 347 | pJRC36 | dscA MS2-tagging vector
| Jonathan Chubb |
| 348 | pJRC76 | MS2-GFP expression vector | Jonathan Chubb |
| 446 | pJRC88 | GFP-Set1:C147A construct (in pDEXH82)
| Jonathan Chubb |
| 447 | pJRC89 | GFP-Set1:N142Q construct (in pDEXH82).
| Jonathan Chubb |
| 458 | pJSK19 | Myc-tagged Dictyostelium replacement Arp2 expressed in pRHI76.
| Mehreen Zaki (Rob Insall) |
| 245 | pKK4 | Tandem affinty purification (TAP) vector.
| Katrin Koch (R. Graf) |
| 450 | pKL2 | ACA fragment from pGSP9 inserted in the Dictyostelium expression vector pB18. pKL2 was used to generate aca-/ACAact-15 cells.
| Carole Parent |
| 485 | pLAI7 | apm4-knockout construct. PCR-amplified 3' and 5' regions of apm4 (mu4 or µ4), with a bsR cassette inserted in between, in pBluescript.
| Francois Letourneur |
| 441 | pLAS103 | Plasmid rescued with EcoRI from cheater mutant LAS103. Remi insertion in gene DDB_G0280089 (unknown function).
| Elizabeth Villegas (Gad Shaulsky) |
| 419 | pLAS18 | Plasmid rescued with EcoRI from cheater mutant LAS18. Non-intragenic REMI insertion near rab7B (Rab GTPase).
| Elizabeth Villegas (Gad Shaulsky) |
| 420 | pLAS19 | Plasmid rescued with EcoRI from cheater mutant LAS19. Remi insertion in pks26 (putative polyketide synthase; beta-ketoacyl synthase family protein).
| Elizabeth Villegas (Gad Shaulsky) |
| 421 | pLAS26 | Plasmid rescued with BglII from cheater mutant LAS26. Remi insertion in DDB_G0277239 (Non-receptor tyrosine kinase spore lysis A (EC 2.7.1.112) (Tyrosine-protein kinase 1).
| Elizabeth Villegas (Gad Shaulsky) |
| 422 | pLAS27 | Plasmid rescued with BglII from cheater mutant LAS27. Non-intragenic remi insertion near DDB_G0280203.
| Elizabeth Villegas (Gad Shaulsky) |
| 423 | pLAS29 | Plasmid rescued with BglII from cheater mutant LAS29. Remi insertion in DDB_G0273201 (DNA-binding HORMA domain-containing protein;
mitotic spindle assembly checkpoint protein).
| Elizabeth Villegas (Gad Shaulsky) |
| 408 | pLAS3 | Plasmid rescued with EcoR1 from cheater mutant LAS3 (DDB0187308; putative ubiquitin-conjugating enzyme E2).
| Elizabeth Villegas (Gad Shaulsky) |
| 424 | pLAS31 | Plasmid rescued with BglII from cheater mutant LAS31. Remi insertion in pikG (phosphatidylinositol 3-kinase; PI3kinase).
| Elizabeth Villegas (Gad Shaulsky) |
| 425 | pLAS32 | Plasmid rescued with BglII from cheater mutant LAS32. Remi insertion in docA (timA, DG1012, DG2016). SH3 domain-containing protein; DOCK family protein;
putative guanine nucleotide exchange factor (GEF). Similar to H. sapiens DOCK180 protein.
| Elizabeth Villegas (Gad Shaulsky) |
| 426 | pLAS34 | Plasmid rescued with BglII from cheater mutant LAS34. Remi insertion in DDB_G0278585 (unknown function).
| Elizabeth Villegas (Gad Shaulsky) |
| 427 | pLAS38 | Plasmid rescued with BglII from cheater mutant LAS38. Non-intragenic REMI insertion (DDB0202322, DDB0202324).
| Elizabeth Villegas (Gad Shaulsky) |
| 428 | pLAS39 | Plasmid rescued with BglII from cheater mutant LAS39. Remi insertion in DDB_G0292148 (Arf GTPase activating protein).
| Elizabeth Villegas (Gad Shaulsky) |
| 429 | pLAS43 | Plasmid rescued with SpeI from cheater mutant LAS43. Remi insertion in DDB_G0274431 (hypothetical 127.0 kDa protein).
| Elizabeth Villegas (Gad Shaulsky) |
| 430 | pLAS44 | Plasmid rescued with SpeI from cheater mutant LAS44. Remi insertion in pks2- (putative polyketide synthase; beta-ketoacyl synthase family protein). | Elizabeth Villegas (Gad Shaulsky) |
| 431 | pLAS49 | Plasmid rescued with BglII from cheater mutant LAS49. Non-intragenic remi insertion near genes DDB_G0273325 and DDB_G0273271.
| Elizabeth Villegas (Gad Shaulsky) |
| 415 | pLAS5 | Plasmid rescued with EcoRI from cheater mutant LAS5. GeneDDB0191519 (rsc6) REMI mutant; similar to heat shock protein Hsp20 domain-containing protein; but does not contain a Hsp20 domain
| Elizabeth Villegas (Gad Shaulsky) |
| 432 | pLAS51 | Plasmid rescued with BglII from cheater mutant LAS51. Non-intragenic REMI insertion near gene DDB_G0285547 (unknown function).
| Elizabeth Villegas (Gad Shaulsky) |
| 434 | pLAS53 | Plasmid rescued with EcoRI from cheater mutant LAS53. Remi insertion in abkD (putative protein serine/threonine kinase;
ABC1 family protein kinase;
ABC1-B subfamily protein kinase). Contains ABC1 kinase (protein kinase-like) domain; yeast ABC1 essential for the electron transfer in the BC(1) complex; E.coli homolog required for ubiquinone biosynthesis). | Elizabeth Villegas (Gad Shaulsky) |
| 433 | pLAS54 | Plasmid rescued with EcoRI from cheater mutant LAS54. Remi insertion in gene DDB_G0280299 (unkown function).
| Elizabeth Villegas (Gad Shaulsky) |
| 435 | pLAS57 | Plasmid rescued with EcoRI from cheater mutant LAS57. Non-intragenic remi insertion between genes DDB_G0268864 and DDB_G0268866 (unknown functions). | Elizabeth Villegas (Gad Shaulsky) |
| 436 | pLAS58 | Plasmid rescued with EcoRI from cheater mutant LAS58. Non-intragenic remi insertion between DDB_G0284973 and tpsB (glycosyltransferase α,α-trehalose-phosphate synthase trehalose 6-phosphate synthase trehalose-phosphatase) | Elizabeth Villegas (Gad Shaulsky) |
| 416 | pLAS6 | Plasmid rescued with EcoRI from cheater mutant LAS6. Remi insertion between genes DDB0203824 (DDB_G0276383) and DDB0201646 (pyr1-3).
| Elizabeth Villegas (Gad Shaulsky) |
| 437 | pLAS60 | Plasmid rescued with ClaI from cheater mutant LAS60. Non-intragenic remi insertion between genes DDB_G0285935 and helD (DEAD/DEAH box helicase;
putative RNA splicing factor). Conserved RNA helicase; S. cerevisiae prp16 involved in the second catalytic step of splicing, exhibits ATP-dependent RNA unwinding activity.
| Elizabeth Villegas (Gad Shaulsky) |
| 417 | pLAS7 | Plasmid rescued with EcoRI from cheater mutant LAS7. Remi insertion in DDB_G0290685 (unknown gene product; highly repetitive protein: contains close to 100 repeats of the sequences DGENNQ, DGGENNQ, or very related sequences)
| Elizabeth Villegas (Gad Shaulsky) |
| 438 | pLAS70 | Plasmid rescued with ClaI from cheater mutant LAS70. Remi insertion in DDB_G0279405 (putative protein serine/threonine kinase;
CAMKK family protein kinase;
Meta subfamily protein kinase).
| Elizabeth Villegas (Gad Shaulsky) |
| 418 | pLAS8 | Plasmid rescued with EcoRI from cheater mutant LAS8. Remi insertion in . Similar to Oryza sativa (Japonica cultivar-group). Putative zinc transporter protein ZIP1.
| Elizabeth Villegas (Gad Shaulsky) |
| 439 | pLAS91 | Plasmid rescued with EcoRI from cheater mutant LAS91. Remi insertion in abcC9 (ABC transporter C family protein).
| Elizabeth Villegas (Gad Shaulsky) |
| 440 | pLAS99 | Plasmid rescued with EcoRI from cheater mutant LAS99. Remi insertion in dhkE (histidine kinase; protein kinase, atypical group; HisK family protein kinase).
| Elizabeth Villegas (Gad Shaulsky) |
| 310 | pLD1A15SN | Modified pLD1 vector which contains the Ddp2-based origin of replication.
The Robinson expression libray is available from the stock center.
| Doug Robinson |
| 298 | pLD1A15SN:dynhp | Expression vector for dynacortin hairpin construct. | Doug Robinson |
| 466 | pLD1A15SN:fimbrin RNAi | Expression vector for fimbrin RNAi hairpin
| Doug Robinson |
| 467 | pLD1A15SN:GFP-fimbrin | Expression vector for GFP-fimbrin
| Doug Robinson |
| 297 | pLD1A15SN:GFPdynacortin |
| Doug Robinson |
| 309 | pLD1A15SN:myoIIhp | Expression vector for myosin II hairpin construct.
| Doug Robinson |
| 122 | pLittle | Autonomous replicating extrachromosomal expression vector with G418 cassette, derived from pBIG by the removal of a 1.3-kb KpnI fragment.
| Tom Egelhoff |
| 9 | pLPBLP | Plasmid containing floxed-Bsr cassette for generating multiple gene disruptions.
| Jan Faix |
| 44 | pMB35 | Transactivator plasmid containing the tTAs* gene under the control of A15P and 2H3T .
| Mieke Blaauw (Peter van Haastert) |
| 45 | pMB38 | Extrachromosomal response plasmid containing the inducible promoter TRE-Pmin upstream of the 2H3T terminator with 4 unique restriction sites in between.
| Mieke Blaauw (Peter van Haastert) |
| 392 | pMBP-Aur | Full-length DdAurora cDNA cloned in bacterial vector pMal-c2X (NEB) for protein purification in E. coli. N-terminal MBP tag; ampR.
| Arturo De Lozanne |
| 19 | pMC25 | cAR1 gene disruption construct (ura selection): 2-kb region immediately upstream of cAR1 coding region and 0.4-kb 3'coding region plus 0.1-kb 3'non-translated region subloned into pBluescript-SK. 3.7-kb ClaI fragment from pDU3B1 was subloned between the fragments.
| Peter Devreotes |
| 20 | pMC34 | cAR1 cDNA minus first 2 codons, (nt 97-1312), cloned into the BglII site of pJK1
| Peter Devreotes |
| 71 | pMC36 | cAR1 cDNA in the BglII site of pJK1
| Peter Devreotes |
| 102 | pMKC-Hyg1 | myosin heavy chain knockout vector containing hygromycin resistance cassette
| Tom Egelhoff |
| 77 | pMYC-86-6 | Vector expressing the piaAWT gene (full-length piaA cDNA). See d6652, PhD Thesis of M.Y. Chen.
| Peter Devreotes |
| 75 | pMYC4 | Plasmid for generating Gα1/Gα2 chimeras (backbone is pBluescript KS-). | Peter Devreotes |
| 76 | pMYC5 | Plasmid for generating Gα2/Gα1 chimeras (backbone is pBluescript KS-).
| Peter Devreotes |
| 323 | pmycELC | ELC (essential myosin light chain) cDNA tagged with a 30-base myc sequence at the 3'end, introduced in expression vector pBORP.
| Rex Chisholm |
| 240 | pN-CAP-Pro-GFP | N-terminal domain of CAP (containing the proline-rich region) cloned in frame with GFP in pDdA15gfp.
| Angelika Noegel |
| 92 | pPIA-HIS-GFP | A PiaA-eGFP expression vector containing a 2x proline linker and the hexyl histidine sequence. A full desription can be found on page 135 of the PhD thesis of J. Silverman (2004).
| Peter Devreotes |
| 222 | pPL3/lacZ | PL3 promoter-lacZ fusion construct (PL3 is prespore gene pspD encoding spore coat protein SP87) | Daphne Blumberg |
| 241 | pPro-C-CAP-GFP | C-terminal domain of CAP (containing the proline-rich region) cloned in frame with GFP in pDdA15gfp.
| Angelika Noegel |
| 354 | pPROF120 | Apoaequorin expression plasmid derived from pDNeo2.
| Paul Fisher |
| 207 | pPROF128 | HspA (chaperonin 60) antisense construct in pDNeo2.
| Paul Fisher |
| 276 | pPsA-Rc | PsA::PKA-Rsubunit (Rc mutant does bind C subunit = control) expression vector
| Adrian Harwood |
| 275 | pPsA-Rm | PsA::PKA-Rsubunit (Rm mutant) expression vector.
| Adrian Harwood |
| 358 | pPT130 | Gateway vector (expression vector for recombination cloning).
| Peter Thomason (Ted Cox) |
| 359 | pPT131 | Gateway vector (expression vector for recombination cloning).
| Peter Thomason (Ted Cox) |
| 360 | pPT132 | Gateway vector (expression vector for recombination cloning).
| Peter Thomason (Ted Cox) |
| 361 | pPT134 | Gateway vector (expression vector for recombination cloning).
| Peter Thomason (Ted Cox) |
| 362 | pPT135 | Gateway vector (expression vector for recombination cloning).
| Peter Thomason (Ted Cox) |
| 363 | pPT165 | Gateway vector (expression vector for recombination cloning).
| Peter Thomason (Ted Cox) |
| 236 | pPT17 | D212N mutant regA "rescue" vector. | Rob Kay |
| 237 | pPT39 | GST-rdeA H63Q expression vector
| Rob Kay |
| 238 | pPT40 | GST-rdeA H65Q expression vector (inactive mutant)
| Rob Kay |
| 287 | pRacF2-G12V | Rho GTPase (RacF2) overexpression vector (constitutive active) | Tetsuya Muramoto (Hideko Ursushihara) |
| 288 | pRacF2-N17T | Rho GTPase (RacF2) overexpression vector (dominant negative).
| Tetsuya Muramoto (Hideko Ursushihara) |
| 286 | pRacF2-WT | Rho GTPase (RacF2) overexpression vector
| Tetsuya Muramoto (Hideko Ursushihara) |
| 5 | pREMI:GFP-1 | REMI plasmid (neoR) for gene trapping, with GFP that can only be expressed from host promoter.
| Petra Fey (Ted Cox) |
| 6 | pREMI:GFP-2 | REMI plasmid (neoR) for gene trapping, with GFP that can only be expressed from host promoter.
| Petra Fey (Ted Cox) |
| 7 | pREMI:GFP-3 | REMI plasmid (neoR) for gene trapping, with GFP that can only be expressed from host promoter.
| Petra Fey (Ted Cox) |
| 12 | pREP | Plasmid for cotransformation with pDXA/DXD series expression vectors. Pedigree: neoR cassette from pnDedeltaI removed by cutting with SacI/BamHI.
| Menno Knetsch (Dietmar Manstein) |
| 303 | pRHI25 | Remi plasmid with truncated pyr5-6. Similar to pRHI30 but some of the PCR'd sequence was removed from the clone.
| Rob Insall |
| 27 | pRHI30 | Plasmid containing a truncated pyr5-6 region.
| Peter Devreotes |
| 84 | pRHI38 | Vector expressing the CRACWT gene. CRAC cDNA cloned into pRHI8, an expression vector derived from pATANB43 (pers. communic. R. Insall, 5/27/08).
| Peter Devreotes |
| 353 | pRHI71 | RasGefA (aimless) in pRHI8, which is a Ddp1-based extrachromosomal vector. | Rob Insall |
| 457 | pRHI76 | Extrachromosomal expression vector. Aequorin inserted as BglII-NotI fragment between the constitutive actin15 promoter and the 2H3 terminator of pRHI8. | Mehreen Zaki (Rob Insall) |
| 459 | pRHI8 | Extrachromosomal expression vector. The BglII and NotI sites are too close to one another so it cuts badly. Use pRHI76 instead.
| |
| 30 | pRJ525 | cAR3 in pBlueScript | Peter Devreotes |
| 83 | pRJ544 (5car3neo) | cAR3 knockout construct
| Peter Devreotes |
| 82 | pRJ648 (5cAR3ura) | cAR3-KO vector
| Peter Devreotes |
| 116 | pRNR-P | regulated expression vector based on the DNA-damage inducibility of the rnrB gene (ribonucleotide reductase promoter)
| Adrian Tsang |
| 478 | pRNR-zyg1 | Inducible expression vector for overexpression of the zyg1 gene. | Aiko Amagai |
| 181 | PSA/idq-galΔ5 | | Harry MacWilliams (through Herb Ennis) |
| 308 | pSadA-GFP | C-terminal GFP fusion
| Petra Fey (Chisholm) |
| 49 | pspA-Gal (D19-Gal) | Construct drives the expression of beta-galactosidase as fusion protein containing the first five amino acids of PsA, under the control of a prespore promoter.
| Jeff Williams |
| 345 | pspA/myc-fbxA | Myc-tagged FbxA under the control of the prespore pspA promoter, in a pDXA |
