| Strain Descriptor |
Strain Summary |
Genotype |
Phenotype |
| [act15]:GFP:myoI(K23333A | constitutively active myoI(K23333A,K2336A) expressed in AX2 | axeA2, axeB2, axeC2, myoI(K23333A, K2336A), [pDTi321], neoR | |
| [act15]:GFP:myoK(801-858) | wild type overexpressing the N-terminally GFP-tagged myoK tail domain (residues 801-858) | axeA2, axeB2, axeC2, [act15]:myoK:Myc, neoR | |
| [act15]:GFP:nek2 | overexpression of nek2 from the actin 15 promoter | axeA2, axeB2, axeC2, [act15]:nek2:GFP | |
| [act15]:GFP:nkaP | nkaP under control of actin promotor overexpressed in AX2 | axeA2, axeB2, axeC2, GFP:nkaP, [pDex79], neoR | |
| [act15]:GFP:numA(1-117:168-340) | overexpressing the short numA isoform (numA1) where the central DEED domain has been deleted in wild type | axeA1, axeB1, axeC1, numA1deltaDEED [pTX-GFP], neoR | |
| [act15]:GFP:nxnB | GFP-nxnB expressed in AX2 | axeA2, axeB2, axeC2, GFP-nxnB, [pDEX-RH79], neoR | |
| [act15]:GFP:pakA | full length PakA marked with GFP at the N terminus, expressed in AX2 cells under control of act15 promotor | axeA2, axeB2, axeC2, GFP:pakA, [pDEXRH], neoR | |
| [act15]:GFP:pakA(797-1197) | catalytic and CRIB domains of PakA marked with GFP at the N-terminus, expressed in AX2 cells under control of act15 promotor | axeA2, axeB2, axeC2, GFP:pakA(797-1197), [pDEXRH], neoR | |
| [act15]:GFP:pats1(2087-2351) | overexpression of pats1 kinase domain (2087-2351) in DH1 | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, [act15]:GFP:pats1(2087-2351), neoR | |
| [act15]:GFP:paxB | paxB expressed in wild type | axeA2, axeB2, axeC2, GFP-paxB, neoR | |
| [act15]:GFP:phg2(1-245) | GFP-phg2 residues 1-245 expression in wild type; uracil auxotroph, neomycin resistant | axeA1, axeB1, axeC1, pyr5-6-[pRG24], ura-, GFP-phg2(1-245)[pFL684]neoR | |
| [act15]:GFP:pldB | AX2 cells expressing pldB fused to GFP under the control of the constitutive act15 promoter | axeA2, axeB2, axeC2, act15:GFP:pldB, [pPT132], neoR | |
| [act15]:GFP:rac1A | overexpressor of GFP-rac1A under the control of the act15 promoter | axeA2, axeB2, axeC2, [act15]:GFP:rac1A, neoR | |
| [act15]:GFP:rac1A(G12V) | rac1A with G12V substitution to make constitutively active in AX2 | axeA2, axeB2, axeC2, rac1A(G12V), [pDGFP-MCS-Neo], neoR | |
| [act15]:GFP:rac1A(T17N) | rac1A with T17N substitution to render inactive in AX2 | axeA2, axeB2, axeC2, rac1A(T17N), [pDGFP-MCS-Neo], neoR | |
| [act15]:GFP:rac1B | overexpressor of GFP(S65T)-rac1B under the control of the act15 promoter | axeA2, axeB2, axeC2, [act15]:GFP:rac1B, neoR | |
| [act15]:GFP:rac1C | overexpressor of GFP(S65T)-rac1C under the control of the act15 promoter | axeA2, axeB2, axeC2, [act15]:GFP:rac1C, neoR | |
| [act15]:GFP:rac1C(G12V) | rac1C with G12V substitution to make constitutively active in AX2 | axeA2, axeB2, axeC2, rac1C(G12V), [pDGFP-MCS-Neo], neoR | |
| [act15]:GFP:rac1C(T17N) | rac1C with T17N substitution to render inactive in AX2 | axeA2, axeB2, axeC2, rac1C(T17N), [pDGFP-MCS-Neo], neoR | |
| [act15]:GFP:racE(G20V) | constitutively GTP-bound GFP-tagged racE with G20V substitution expressed in AX2 | axeA2, axeB2, axeC2, racE(G20V), [pTX-GFP], neoR | |
| [act15]:GFP:racE(G20V/S192A) | constitutively GTP-bound, phospho-defective GFP-tagged racE with (G20V/S192A) substitutions expressed in AX2 | axeA2, axeB2, axeC2, racE(G20V/S192A), [pTX-GFP], neoR | |
| [act15]:GFP:racE(G20V/S192A/E180R/R186E) | constitutively GTP-bound, phospho-defective GFP-tagged racE with (G20V/S192A/E180R/R186E)
substitutions expressed in AX2 | axeA2, axeB2, axeC2, racE(G20V/S192A/E180R/R186E), [pTX-GFP], neoR | |
| [act15]:GFP:racE(T25N) | constitutively GDP-bound, GFP-tagged racE(T25N) expressed in AX2 | axeA2, axeB2, axeC2, racE(T25N), [pTX-GFP], neoR | |
| [act15]:GFP:racE(T25N/E173R/R186E/S192D) | constitutively GDP-bound, phospho-mimetic, GFP-tagged racE(T25N/E173R/R186E/S192D)
expressed in AX2 | axeA2, axeB2, axeC2, racE(T25N/E173R/R186E/S192D), [pTX-GFP], neoR | |
| [act15]:GFP:racE(T25N/E180R/S192D) | constitutively GDP-bound, phospho-mimetic, GFP-tagged racE(T25N/E180R/S192D)
expressed in AX2 | axeA2, axeB2, axeC2, racE(T25N/E180R/S192D), [pTX-GFP], neoR | |
| [act15]:GFP:racF2 | Overexpression in V12 of GFP:RacF2 under the act15 promoter | matA2, [pRacF2-WT], neoR | neoR,aggregation defective |
| [act15]:GFP:racF2 | Overexpression of GFP:RacF2 in KAX3 under the actin 15 promoter. | axeA1, axeB1, axeC1, [pRacF2-WT], neoR | axe,neoR |
| [act15]:GFP:racG(1-168):racH(164-200(D186A/D191A/D193A/D196A)) | chimera of racG (residues 1 to 168) and racH (residues 164 to 200) with 4 point mutations (D to A) at positions 186, 191, 193 and 196, fused with GFP at the amino terminus | axeA2, axeB2, axeC2, [act15/GFP:racG(1-168):racH(164-200(D186A/D191A/D193A/D196A))], neoR | |
| [act15]:GFP:racG(1-168):racH(164-200) | chimera of racG (residues 1 to 168) and racH (residues 164 to 200), fused with GFP at the amino terminus | axeA2, axeB2, axeC2, [act15/GFP:racG(1-168):racH(164-200)], neoR | |
| [act15]:GFP:racG(C183S) | racG(C183S) mutant fused with GFP at the amino terminus | axeA2, axeB2, axeC2, [act15]:GFP:racG(C183S), neoR | |
| [act15]:GFP:racH | overexpressor of racH fused with GFP at amino terminus | axeA2, axeB2, axeC2, [act15]:GFP:racH, neoR | |
| [act15]:GFP:racH(1-163):racG(167-201) | chimera of racH (residues 1 to 163) and racG (residues 164 to 201), fused with GFP at the amino terminus | axeA2, axeB2, axeC2, [act15/GFP:racH(1-163):racG(167-201)], neoR | |
| [act15]:GFP:racH(D186A/D191A/D193A/D196A) | racH with 4 point mutations (D to A) at positions 186, 191, 193 and 196, fused with GFP at the amino terminus | axeA2, axeB2, axeC2, [act15/GFP:racH(D186A/D191A/D193A/D196A)], neoR | |
| [act15]:GFP:racH(M13V) | constitutively active mutant of racH, fused with GFP at the amino terminus | axeA2, axeB2, axeC2, [act15/GFP:racH(M13V)], neoR | |
| [act15]:GFP:racH(Q200M) | racH(Q200M) mutant fused with GFP at the amino terminus | axeA2, axeB2, axeC2, [act15/GFP:racH(Q200M)], neoR | |
| [act15]:GFP:racH(T18N) | dominant negative mutant of racH fused with GFP at the amino terminus | axeA2, axeB2, axeC2, [act15/GFP:racH(T18N)], neoR | |
| [act15]:GFP:racH:gemA(630-658) | fusion of racH with mitochondrial targeting region of gemA (Miro), fused with GFP at the amino terminus | axeA2, axeB2, axeC2, [act15/GFP:racH:gemA(630-658)], neoR | |
| [act15]:GFP:rapA | overexpressor of rapA fused with GFP under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15/GFP:rapA], neoR | |
| [act15]:GFP:rapA(G12V) | overexpressor of a constitutively active form of rapA fused with GFP under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15/GFP:rapA(G12V)], neoR | |
| [act15]:GFP:rapA(S17N) | overexpressor of a dominant negative form of rapA fused with GFP under the control of the act15 promoter | axeA1, axeB1, axeC1, [act15/GFP:rapA(S17N)], neoR | |
| [act15]:GFP:rheb(D60I) | dominant-negative, non-GTP-bound Rheb with D60I substitution marked with GFP and expressed in AX3 | axeA1, axeB1, axeC1, [GFP:rheb(D60I)], neoR | |
| [act15]:GFP:rheb(Q64L) | dominant active, GTP-bound Rheb with Q64L substitution, marked with GFP and expressed in AX3 | axeA1, axeB1, axeC1, [GFP:rheb(Q64L)], neoR | |
| [act15]:GFP:sbds | GFP-sbds fusion expressed under the control of the act15 promoter; neomycin resistant | axeA2, axeB2, axeC2, [GFP-SBDS], neoR | |
| [act15]:GFP:sbds | GFP-sbds fusion expressed under the control of the actin 15 promoter; neomycin resistant | axeA2, axeB2, axeC2, [GFP-SBDS], neoR | |
| [act15]:GFP:secG | GFP-secG overexpressed in wild type | axeA2, axeB2, axeC2, GFP-secG, neoR | |
| [act15]:GFP:sey1 | N-terminal GFP-tagged Sey1 in AX3 | axeA1, axeB1, axeC1, sey1, [pDM317], neoR | |
| [act15]:GFP:sey1(K154A) | N-terminal GFP-tagged Sey1 with K154A substitution to render catalytically inactive in AX3 | axeA1, axeB1, axeC1, sey1(K154A), [pDM317], neoR | |
| [act15]:GFP:src1(2-646) | GFP-tagged src1(2-646) overexpressed in AX2 | axeA2, axeB2, axeC2, GFP:src192-646), neoR | |
| [act15]:GFP:sun1(283-905) | N-terminal deletion (1-282) construct overexpressed in wild type | axeA2, axeB2, axeC2, GFP-sun1(283-905), neoR | |
| [act15]:GFP:talA/mhcA-/talA- | N-terminal GFP-tagged talA expressed in a mhcA/talA double null strain | axeA1, axeB1, axeC1, pyr5-6-[PYR5-6-SalI-linker], thy-[PYR5-6-THY1], ura+, thy+, mhcA-[pTZD1THY], talA-, GFP:talA, bsR, neoR | |
| [act15]:GFP:vatB | N-terminal GFP-vatB fusion over-expressed in AX2 | axeA2, axeB2, axeC2, neoR, [actin15-vatB::GFP] | axe, neoR |
| [act15]:gol(76-118):GFP | Core fragment Δ(1-75;119-579)-golvesin(C)-GFP expressing cells | axeA2, axeB2, axeC2, gol(76-118):GF[pDEX], neoR | axe, neoR, gfp |
| [act15]:gol(76-579):GFP | N-terminally deleted GFP-(N)-Δ(1-75)-golvesinGFP expressing cells | axeA2, axeB2, axeC2, gol(76-579):GFP, [pDEX], neoR | axe, neoR, gfp |
| [act15]:gol:GFP | gol (golvesin) fused to GFP at the carboxyl terminus; trapped in Golgi apparatus | axeA2, axeB2, axeC2, [act15/golvesin-gfp], neoR | axe, neoR, gfp |
| [act15]:gpaA | overexpressor of gpaA under the control of the constitutive act15 promoter | [act15]:gpaA, neoR | |
| [act15]:gpaB | gpaB overexpressed in KAX3 | axeA1, axeB1, axeC1, gpaB, [pExp4(+)], neoR | |
| [act15]:gpaB(K12A/L19A/I21A) | gpaB(K12A/L19A/I21A) expressed in KAX3 | axeA1, axeB1, axeC1, gpaB(K12A/L19A/I21A), [pJH1075], bsR | |
| [act15]:gpaE | GpaE overexpressed in KAX3 | axeA1, axeB1, axeC1, gpaE, [pTX-GFP], neoR | |
| [act15]:gpaG | gpaG (G alpha 7) overexpressor | axeA1, axeB1, axeC1, [act15]:gpaG, neoR | |
| [act15]:gpaH | gpaH (G alpha 8) overexpressor | axeA1, axeB1, axeC1, [act15]:gpaH, neoR | |
| [act15]:gpaH/AX2 | gpaH under control of act15 promotor overexpressed in AX2 | axeA2, axeB2, axeC2, gpaH, [pDM304], neoR | |
| [act15]:gpaI(G38V):FLAG | gpaI(G38V), FLAG-tagged overexpression in wild type cells, constitutively active mutant; neomycin resistant | axeA1, axeB1, axeC1, [gpaI(G38V)], neoR | |
| [act15]:gpaI(Q196L):FLAG | gpaI(Q196L), FLAG-tagged overexpression in wild type cells, constitutively active mutant; neomycin resistant | axeA1, axeB1, axeC1, [gpaI(Q196L)], neoR | |
| [act15]:gppA:GFP | AX2, P29F8-S65T-GFP (AX2 cells expressing dajumin-GFP) | axeA2, axeB2, axeC2, gppA:GFP, [pDEXH], neoR | axe, neoR, gfp |
| [act15]:gppA:GFP | AX2, P29F8-S65T-GFP (AX2 cells expressing dajumin-GFP) | axeA2, axeB2, axeC2, gppA:GFP, [pDEXH], neoR | axe, neoR, gfp |
| [act15]:grlA:GFP | grlA-GFP overexressed in wild type AX2 | axeA2, axeB2, axeC2, grlA-GFP, bsR | |
| [act15]:grlB | grlB over expressed in AX2 cells | axeA2, axeB2, axeC2, grlB, neoR | |
| [act15]:grxA | grxA overexpressed in kAX3 under the control of the act15 promoter | axeA1, axeB1, axeC1, neoR, grxA-OE | delayed culmination |
| [act15]:grxA(C21S) | grxA-C21S (catalytic dead) overexpressed in KAX3 | axeA1, axeB1, axeC1, neoR, [act15]:grxA(C21S) | wild type |
| [act15]:grxA(C21S) | overexpression of catalytically inactive grxA under the control of the act15 promoter | axeA1, axeB1, axeC1, neoR, [act15]:grxA(C21S) | |
| [act15]:grxA/[act15]:lacZ | grxA overexpressed in KAX3 under the control of the act15 promoter, marked with lacZ | axeA1, axeB1, axeC1, neoR, [act15]:grxA, [act15]:lacZ | |
| [act15]:gshB | gshB overexpressed in KAX3 | axeA1, axeB1, axeC1, gshB, [pExp4(+)], neoR | |
| [act15]:gshB/[AS]gpaB | antisense gpaB mutant in gshB overexpressed in KAX3 | axeA1, axeB1, axeC1, gshB, [pExp4(+)], [pDBsrXP-GFP], bsR, neoR | |
| [act15]:gsr | gsr overexpressed in kAX3 | axeA1, axeB1, axeC1, neoR, [act15]:gsr | delayed culmination |
| [act15]:gst4 | gst4 overexpressed in AX2 | axeA2, axeB2, axeC2, gst4, [pHK12neo], neoR | |
| [act15]:HA:ppp4C/[act15]:V5:smkA | HA-tagged ppp4C and V5-tagged smkA coexpressed in wild type | axeA1, axeB1, axeC1, V5:smkA+ [pEXP4(+)], HA: ppp4C + [pEXP4(+)], neoR | |
| [act15]:HA:rab11(N126I) | HA-tagged rab11 with N126I substitution in AX4 | axeA1, axeB1, axeC1, HA:rab11(N126I), [pHA80], neoR | |
| [act15]:HA:Rab14(N121I) | overexpressor of rab14 (N121I) (dominant negative) with a N-terminal HA tag | axeA1, axeB1, axeC1, [act15]:Rab14(N121I), neoR | |
| [act15]:HA:rab7A(Q67L) | constitutively active HA-tagged rab7A with Q67L substitution overexpressed in AX4 | axeA1, axeB1, axeC1, HA:rab7A(Q67L), [pDA80-HA], neoR | |
| [act15]:HA:rab7A(T22N) | dominant negative HA-tagged rab7A with T22N substitution overexpressed in AX4 | axeA1, axeB1, axeC1, HA:rab7A(T22N), [pDA80-HA], neoR | |
| [act15]:HA:smkA | HA-tagged smkA overexpressed in wild type | axeA1, axeB1, axeC1, HA:smkA+ [pEXP4(+)], neoR | |
| [act15]:hcpA(1-161:218-225):GFP | overexpression of a deletion of the hcpA C-terminal chromo shadow domain; C-terminal GFP fusion | axeA2, axeB2, axeC2, hcpA-[hcpAdeltaC-GFP], neoR | axe, neoR; slow growth; reduced viability |
| [act15]:hcpA:GFP | C-terminal hcpA-GFP fusion under the control of the act15 promoter expressed in wt | axeA2, axeB2, axeC2, hcpA-[hcpA-GFP], neoR | axe,neoR |
| [act15]:hcpB(1-197:237-244):GFP | overexpression of a deletion of the hcpB C-terminal chromo shadow domain; C-terminal GFP fusion | axeA2, axeB2, axeC2, hcpB-[hcpBdeltaC-GFP], neoR | axe, neoR; slow growth; |
| [act15]:hcpB:GFP | C-terminal HcpB-GFP fusion under the control of the act15 promoter expressed in wt | axeA2, axeB2, axeC2, hcpA-[hcpB-GFP], neoR | axe,neoR |
| [act15]:HIS:tgrB1/AX4 | secretory overexpression extracellular region of TgrB1; N-term His marker | axeA1, axeB1, axeC1, tgrB1(AX4), [pDXA-A15::ssHis], neoR | |
| [act15]:His:tgrB1/QS31 | secretory overexpression of the extracellular region of TgrB1 (QS31); N-term His marker | axeA1, axeB1, axeC1, tgrB1(QS31), [pDXA-A15ssHis7], neoR | |
| [act15]:His:tgrB1/QS37 | Secretory overexpression of the extracellualar region of tgrB1(QS37); N-term His marker | axeA1, axeB1, axeC1, tgrB1(QS37), [pDXA-A15:ssHis], neoR | |
| [act15]:His:tgrB1/QS4 | Secretory overexpression of the extracellular region of TgrB1 (QS4); N-term His marker | axeA1, axeB1, axeC1, tgrB1(QS4), [pDXA-A15ssHis], neoR | |
| [act15]:His:tgrB1/QS45 | secretory overexpression of the extracellular region of tgrB1(QS45); N-term His marker | axeA1, axeB1, axeC1, tgrB1(QS45), [pDXA-A15:ssHis], neoR | |
| [act15]:hsp101(K213A) | dominant-negative hsp101(K613A) in AX2 | axeA2, axeB2, axeC2, hsp101(K213A), [pDM353], neoR | |
| [act15]:hsp101(K613A) | dominant-negative hsp101(K613A) in AX2 | axeA2, axeB2, axeC2, hsp101(K613A), [pDM353], neoR | |
| [act15]:hspA(1-150):GFP | hspA (amino acids 1 to 150) fused to GFP under the control of the act15 promoter | axeA2, axeB2, axeC2, [pPROF298], neoR | |
| [act15]:hspA(1-23):GFP | hspA (amino acids 1 to 23) fused to GFP under the control of the act15 promoter | axeA2, axeB2, axeC2, [pPROF294], neoR | |
| [act15]:hspA(1-40):GFP | hspA (amino acids 1 to 40) fused to GFP under the control of the act15 promoter | axeA2, axeB2, axeC2, [pPROF295], neoR | |
| [act15]:hssA | hssA overexpressor mutant under the control of the act15 promoter | axeA2, axeB2, axeC2, [OE]hssA- | |
| [act15]:htrA(S300A) | htrA with S300A substitution ablating catalytic activity expresssed in AX2 | axeA2, axeB2, axeC2, htrA(S300A), [pPROF267], neoR | |
| [act15]:htt:eYFP | C-terminal eYFP-marked htt under control of act15 promotor in AX2 | axeA2, axeB2, axeC2, htt, neoR | |
| [act15]:htt:GFP | overexpressing GFP tagged htt strain in AX2 | axeA2, axeB2, axeC2, [pJK1-htt:GFP], neoR | |
| [act15]:ibrA:GFP | wild type cells expressing ibrA-GFP | axeA1, axeB1, axeC1, ibrA:GFP, hygR | |
