Chemotaxis Practical
Compilation of the comments and suggestions received
by Thierry Soldati concerning ways to use Dictyostelium chemotaxis assays
in teaching labs.
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Protocols
These are pdf files.
Comments from Researchers
References suggested by contributors
- Edelstein-Keshet,
L (1988) Mathematical Models in Biology, McGraw
Hill, (originally published by Random House/Birkhauser)
ISBN 394-35507-5
- Finney
R, Varnum B, Soll DR. 1979. "Erasure"
in Dictyostelium: a dedifferentiation involving the programmed loss of
chemotactic functions. Dev Biol. 73(2):290-303.
PMID: 499669
- Gauthier
ML, O'Day DH, 2001. Detection of calmodulin-binding proteins and calmodulin-dependent
phosphorylation linked to calmodulin-dependent chemotaxis to folic and cAMP in Dictyostelium.
Cell Signal 13(8):575-84.
PMID: 11483410
- Hadwiger,J.A.,and
J.Srinivasan, 1999. Folic acid stimulation of the Ga 4 G protein-mediated signal transduction
pathway inhibits anterior prestalk cell development in Dictyostelium. Differentiation
64:195-204.
PMID: 10408952
- Konijn TM, Van Haastert PJ. 1987. Measurement of chemotaxis in Dictyostelium.
Methods Cell Biol. 1987;
28:283-98.
PMID: 3600409
- Kuwayama
H, Ishida S, Van Haastert PJ. 1993. Non-chemotactic Dictyostelium discoideum
mutants with altered cGMP signal transduction.
J
Cell Biol.
1993 Dec;123(6 Pt 1):1453-62.
PMID: 7902839
- O'Day,
D.H.,
1979. Aggregation during sexual development in Dictyostelium discoideum. Can.
J. Microbiol. 25: 1416-1426.
PMID: 231480
- Parent
C.A. 2000. Dictyostelium cell dynamics, Current Protocols in Cell Biology
(J.S. Bonifacino, M. Dasso, J. Lippincott-Schwartz, J.B. Harford, K.M. Yamada;
Eds. John Wiley & Sons Press), Vol. 1, Unit 12.5
- Rifkin
JL. 2002. Quantitative analysis
of the behavior of Dictyostelium discoideum amoebae: stringency of
pteridine reception.
Cell Motil Cytoskeleton.
51(1):39-48.
PMID 11810695
- Margaret
Nelson’s web site: http://merlin.alleg.edu/employee/m/mnelson/courses/Bio290/Dicty.html
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