Chemotaxis Practical

Chemotaxis Practical

Compilation of the comments and suggestions received by Thierry Soldati concerning ways to use Dictyostelium chemotaxis assays in teaching labs.


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Protocols

These are pdf files.


Comments from Researchers


References suggested by contributors

  • Edelstein-Keshet, L (1988) Mathematical Models in Biology, McGraw Hill, (originally published by Random House/Birkhauser)
    ISBN 394-35507-5

  • Finney R, Varnum B, Soll DR. 1979.  "Erasure" in Dictyostelium: a dedifferentiation involving the programmed loss of chemotactic functions. Dev Biol. 73(2):290-303.
    PMID: 499669

  • Gauthier ML, O'Day DH, 2001. Detection of calmodulin-binding proteins and calmodulin-dependent phosphorylation linked to calmodulin-dependent chemotaxis to folic and cAMP in Dictyostelium. Cell Signal 13(8):575-84.
    PMID: 11483410

  • Hadwiger,J.A.,and J.Srinivasan, 1999. Folic acid stimulation of the Ga 4 G protein-mediated signal transduction pathway inhibits anterior prestalk cell development in Dictyostelium. Differentiation 64:195-204.
    PMID: 10408952

  • Konijn TM, Van Haastert PJ. 1987.  Measurement of chemotaxis in Dictyostelium. Methods Cell Biol. 1987; 28:283-98.
    PMID: 3600409

  • Kuwayama H, Ishida S, Van Haastert PJ. 1993. Non-chemotactic Dictyostelium discoideum mutants with altered cGMP signal transduction. J Cell Biol. 1993 Dec;123(6 Pt 1):1453-62.
    PMID: 7902839

  • O'Day, D.H., 1979. Aggregation during sexual development in Dictyostelium discoideum. Can. J. Microbiol. 25: 1416-1426.
    PMID: 231480

  • Parent C.A. 2000. Dictyostelium cell dynamics, Current Protocols in Cell Biology (J.S. Bonifacino, M. Dasso, J. Lippincott-Schwartz, J.B. Harford, K.M. Yamada; Eds. John Wiley & Sons Press), Vol. 1, Unit 12.5

  • Rifkin JL. 2002. Quantitative analysis of the behavior of Dictyostelium discoideum amoebae: stringency of pteridine reception. Cell Motil Cytoskeleton. 51(1):39-48.
    PMID 11810695

  • Margaret Nelsonís web site: http://merlin.alleg.edu/employee/m/mnelson/courses/Bio290/Dicty.html

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