Chemotaxis Practical

Chemotaxis Practical

Compilation of the comments and suggestions received by Thierry Soldati concerning ways to use Dictyostelium chemotaxis assays in teaching labs.



These are pdf files.

Comments from Researchers

References suggested by contributors

  • Edelstein-Keshet, L (1988) Mathematical Models in Biology, McGraw Hill, (originally published by Random House/Birkhauser)
    ISBN 394-35507-5

  • Finney R, Varnum B, Soll DR. 1979.  "Erasure" in Dictyostelium: a dedifferentiation involving the programmed loss of chemotactic functions. Dev Biol. 73(2):290-303.
    PMID: 499669

  • Gauthier ML, O'Day DH, 2001. Detection of calmodulin-binding proteins and calmodulin-dependent phosphorylation linked to calmodulin-dependent chemotaxis to folic and cAMP in Dictyostelium. Cell Signal 13(8):575-84.
    PMID: 11483410

  • Hadwiger,J.A.,and J.Srinivasan, 1999. Folic acid stimulation of the Ga 4 G protein-mediated signal transduction pathway inhibits anterior prestalk cell development in Dictyostelium. Differentiation 64:195-204.
    PMID: 10408952

  • Konijn TM, Van Haastert PJ. 1987.  Measurement of chemotaxis in Dictyostelium. Methods Cell Biol. 1987; 28:283-98.
    PMID: 3600409

  • Kuwayama H, Ishida S, Van Haastert PJ. 1993. Non-chemotactic Dictyostelium discoideum mutants with altered cGMP signal transduction. J Cell Biol. 1993 Dec;123(6 Pt 1):1453-62.
    PMID: 7902839

  • O'Day, D.H., 1979. Aggregation during sexual development in Dictyostelium discoideum. Can. J. Microbiol. 25: 1416-1426.
    PMID: 231480

  • Parent C.A. 2000. Dictyostelium cell dynamics, Current Protocols in Cell Biology (J.S. Bonifacino, M. Dasso, J. Lippincott-Schwartz, J.B. Harford, K.M. Yamada; Eds. John Wiley & Sons Press), Vol. 1, Unit 12.5

  • Rifkin JL. 2002. Quantitative analysis of the behavior of Dictyostelium discoideum amoebae: stringency of pteridine reception. Cell Motil Cytoskeleton. 51(1):39-48.
    PMID 11810695

  • Margaret Nelsonís web site:


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