Genes Identified by Homologous Recombination

Locus

Gene Product

Recent Reference

Process affected in null mutants

abpA

alpha actinin

1

Witke et al. (1992)

culmination

abpC

actin geletation: ABP120

1

Witke et al. (1992)

motility, culmination

acaA 

adenylyl cyclase

3

Pitt et al.  (1992)

cAMP response during aggregation

acgA

adenylyl cyclase

5

van Es et al.. (1996)

osmosensitive germination

aleA 

ras GEF

2

Insall et al. (1996)

chemotaxis

camA

calmodulin

6

Liu et al. (1992)

cytokinesis

capA

cAMP binding protein

2

Bain and Tsang (1991)

growth and development

capB

cAMP binding protein

2

Bain and Tsang (1991)

growth and development

carA

cAMP receptor  cAR1

2

Milne et al. (1997)

cAMP response during aggregation

carB

cAMP receptor  cAR2

5

Saxe et al. (1993)

tip formation

carC

cAMP receptor  cAR3

3

Johnson et al. (1993)

none

carD

cAMP receptor  cAR4

3

Louis et al. (1994)

culmination

chcA

clathrin heavy chain

2

Niswonger and O'Halloran (1997)

pinocytosis; spore formation

cmfA

conditioned media factor

2

Yuen et al. (1995)

aggregation

corA

coronin

1

de Hostos et al. (1993)

cytokinesis, motility

cotA

spore coat protein 96

2

Fosnaugh et al.(1994)

porous spore coats

cotB

spore coat protein 70

2

Fosnaugh et al. (1994)

porous spore coats

cotC

spore coat protein 60

2

Fosnaugh et al. (1994)

porous spore coats

csaA

gp80

5

Noegel et al.  (1986)

cell adhesion

csbA-C

p12

2

Loomis and Fuller (1991)

delayed contact sites B

cycB

cyclinB

2

Luo et al. (1994)

growth

dagA

CRAC

4

Insall et al. (1994)

aggregation

dhkA

histidine kinase

6

Wang et al. (1996)

sporulation

dscA

discoidin

2

Alexander et al. (1992)

cell shape

ecmA

extracell. matrix protein ST430

3

Morrison et al.. (1994)

slug shape

ecmB

extracell. matrix protein ST310

2

Morrison et al. (1994)

none

erkB 

extracellular response kinase 2

4

Segall et al. (1995)

aggregation

fusB

sexual fusion protein gp138B

5

Yamaguchi et al. (1996)

zygote fusion

gbfA

G-box binding factor

5

Schnitzler et al. (1994)

cell type divergence

glpD

glycogen phosphorylase

5

Rogers et al. (1994)

glycogen metabolism

gluA

beta glucosidase

6

Bush et al. (1994)

none

gpaA

G-protein subunit alpha 1

4

Devreotes (1994)

none

gpaB

G-protein subunit alpha 2

2

Devreotes (1994)

aggregation

gpaD

G-protein subunit alpha 4

4

Hadwiger et al. (1994)

folate responses

gpaE

G-protein subunit alpha 5

4

Hadwinger et al. (1996)

tip formation

gpaF

G-protein subunit alpha 6

4

Devreotes (1994)

none

gpaG

G-protein subunit alpha 7

3

Wu et al. (1994)

none

gpaH

G-protein subunit alpha 8

4

Wu et al. (1994)

none

gpbA

G-protein subunit beta

2

Wu ET AL. (1995)

aggregation

ksnG

kinesin 7

3

DeHostos (unpubl)

rapid development

lagC 

gp150

3

Dynes et al. (1994)

mound

manA

alpha mannosidase

6

Schatzle et al. (1991)

none

mhcA

myosin heavy chain

4

Spudich (1989)

cytokinesis, motility

mlcE

myosin light chain

2

Pollenz et al. (1992)

cytokinesis, motility

mlcR

myosin light chain

4

Chen et al. (1994)

cytokinesis, motility

myoA

myosin IA

3

Titus et al. (1993)

chemotaxis

myoB

myosin IB

5

Temesvari et al. (1996)

chemotaxis, secretion

myoC

myosin IC

2

Temesvari et al. (1996)

chemotaxis, secretion

myoD

myosin ID

2

Jung et al., (1996)

growth, motility

myoF

myosin IF

5

Titus et al. (1995)

motility

nagA

N-acetylglucosaminidase

4

Graham et al. (1988)

none

ndkA

nucelotide diphosphate kinase

3

Aguado-Velasco et al. (1996)

cell shape

nxnA

annexin VII

1

Doring et al. (1991)

membrane fusion, secretion

pdiA

cAMP phosphodiesterase inhibitor

3

Franke and Kessin (1992)

aggregation

pdsA

cAMP phosphodiesterase

4

Franke and Kessin (1992)

aggregation

pkaC

protein kinase A catalytic subunit

4

Wang and Kuspa (1997)

aggregation etc.

pkaR

protein kinase A regulatory subunit

3

Harwood et al. (1992)

rapid development

ponA

ponticulin

1

Hitt, A. et al. (1994)

loss of membrane mediated actin nucleation

proB

profilin II

4

Haugwitz et al. (1994)

growth, motility, increased F-actin ( with proA-)

ptpA

phosphotyrosine phosphatase ptp1

2

Howard et al. (1993)

cell shape, stalk formation

ptpC

phosphotyrosine phosphatase ptp3

2

Gamper et al. (1996)

morphogenesis

pyr5-6

UMP-synthetase

3

Jacquet et al. (1988)

uracil auxotroph

rasD

ras

6

Louis et al. (1997)

cell fate

rasG

ras

1

Khosla et al. (1996)

aggregation

rtoA 

--

2

Wood et al. (1996)

coupling of cell types to cell cycle

sevA

severin

5

Andre et al.  (1989)

none

smlA

--

4

Brock et al. (1996)

aggregation

spiA

spore coat protein

5

Richardson et al. (1991)

spore impairment

splA

tyrosine kinase

4

Nuckolls et al. (1996)

sporulation

tagB

serine protease/ ABCP

4

Shaulsky et al. (1995)

prestalk signaling

tipA

--

3

Stege et al. (1997)

prestalk sorting

thyA

--

3

Dynes and Firtel (1989)

thymine auxotrophy

uppA

UDPG pyrophosphorylase

1

Dimond et al. (1976)

ECM of the slug

yelA

--

3

Osherov et al. (1997)

rapid development

wacA

MIP family (aquaporin-like)

3

Flick et al. (1997)

none