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Inaba, Hironori, Yoda, Koji, Adachi, Hiroyuki, (2017) ' The F-actin-binding RapGEF GflB is required for efficient macropinocytosis in Dictyostelium. ' J. Cell. Sci.
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Abstract:Macropinocytosis involves the uptake of large volumes of fluid, which is regulated by various small GTPases. The Dictyostelium discoideum protein GflB is a guanine nucleotide exchange factor (GEF) of Rap1, involved in chemotaxis. Here we studied the role of GflB in macropinocytosis, phagocytosis, and cytokinesis. In plate culture of vegetative cells, compared with the parental strain AX2, gflB KO cells were flatter and more polarized, whereas GflB-overproducing cells were rounder. The gflB KO cells exhibited impaired crown formation and retraction, particularly retraction, resulting in more crowns (macropinocytic cups) per cell and longer crown lifetimes. Accordingly, gflB KO cells showed defects in macropinocytosis and also in phagocytosis and cytokinesis. F-actin was elevated in gflB KO cells. GflB localized to the actin cortex especially at crowns and phagocytic cups. The villin headpiece domain (VHP)-like N-terminal domain of GflB directly interacted with F-actin in vitro Furthermore, a domain enriched in basic amino acids interacted with specific membrane cortex structures such as the cleavage furrow. In conclusion, GflB acts as a key local regulator of actin-driven membrane protrusion possibly by modulating Rap1 signaling pathways.
Status: aheadofprint Type: Journal article Source: PUBMED PubMed ID: 28778987

 
Genes addressed in this paper
act1 act10 act11 act12 act13 act14 act15 act16
Topics in this paper
Mitosis







Mammalian Gene Related







Protein Functional Domain







Cytoskeleton X X X X X X X X
Strains/Constructs







Cellular Location







Function/Process







Endocytosis







Protein-Protein Interactions X X X X X X X X
Regulatory Role







Regulated By







Mutants/Phenotypes









Genes addressed in this paper
act19 act2 act20 act21 act22 act3 act4 act5
Topics in this paper
Mitosis







Mammalian Gene Related







Protein Functional Domain







Cytoskeleton X X X X X X X X
Strains/Constructs







Cellular Location







Function/Process







Endocytosis







Protein-Protein Interactions X X X X X X X X
Regulatory Role







Regulated By







Mutants/Phenotypes









Genes addressed in this paper
act6 act7 act8 act9 gflB rapA
Topics in this paper
Mitosis



X
Mammalian Gene Related



X X
Protein Functional Domain



X
Cytoskeleton X X X X X
Strains/Constructs



X
Cellular Location



X
Function/Process



X
Endocytosis



X
Protein-Protein Interactions X X X X X
Regulatory Role



X X
Regulated By




X
Mutants/Phenotypes



X