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Lohia, Rakhee, Jain, Punita, Jain, Mukul, Mishra, Himanshu, Burma, PK Pradeep Kumar, Shrivastava, Anju, Saran, Shweta, (2018) ' Deletion of Dictyostelium discoideum Sir2A impairs cell proliferation and inhibits autophagy. ' J. Biosci. 43 351-364

 

Abstract:Sirtuins are a family of deacetylases (Class III histone deacetylases) with evolutionarily conserved functions in cellular metabolism and chromatin regulation. Out of the seven human Sirtuins, the function of Sirt2 is the least understood. The purpose of the present study was to investigate the role of Sir2A, a homolog of human Sirt2 in Dictyostelium discoideum (Dd), a lower eukaryote. We created both overexpressing and deletion strains of Ddsir2A to analyse its functions. We observed sir2A mRNA expression throughout development and the transcript was present in the prespore/spore region of multicellular structures developed. They show a preference towards prestalk/stalk pathway when co-developed with wildtype cells during chimera formation. Deletion strain showed a multi-tipped phenotype, decrease in cell proliferation and inhibition of autophagy. In conclusion, our results show low cAMP levels, reduced cell-adhesion, weak cell migration and impaired autophagy to be responsible for the phenotype shown by the null cells. This study provides new insights into the functions of Ddsir2A.
Status:	ppublish	Type:	Journal article	Source:	PUBMED	PubMed ID:	29872023

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	Genes addressed in this paper
	atg1	atg18	atg5	atg8a	atg8b	atg9	cadA	csaA
Topics in this paper
Mammalian Gene Related
Non-mammalian Gene Related
Cellular Location
Function/Process
Transcriptional Regulation	X	X	X	X	X	X	X	X
RNA Levels and Processing	X	X	X	X	X	X	X	X
Cell Type Localization
Protein Physical Properties
Protein Functional Domain
Strains/Constructs
Development/Morphogenesis
Chemotaxis/Motility
Phylogenetic Analysis
Regulated By	X	X	X	X	X	X	X	X
Growth
Mutants/Phenotypes

	Genes addressed in this paper
	sir2A	sir2B	sir2C	sir2D	sir2E	tipD
Topics in this paper
Mammalian Gene Related	X	X	X	X	X
Non-mammalian Gene Related	X	X	X	X	X
Cellular Location	X
Function/Process	X
Transcriptional Regulation						X
RNA Levels and Processing	X					X
Cell Type Localization	X
Protein Physical Properties	X	X	X	X	X
Protein Functional Domain	X	X	X	X	X
Strains/Constructs	X
Development/Morphogenesis	X
Chemotaxis/Motility	X
Phylogenetic Analysis	X	X	X	X	X
Regulated By						X
Growth	X
Mutants/Phenotypes	X