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Lohia, Rakhee, Jain, Punita, Jain, Mukul, Mishra, Himanshu, Burma, PK Pradeep Kumar, Shrivastava, Anju, Saran, Shweta, (2018) ' Deletion of Dictyostelium discoideum Sir2A impairs cell proliferation and inhibits autophagy. ' J. Biosci. 43 351-364
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Abstract:Sirtuins are a family of deacetylases (Class III histone deacetylases) with evolutionarily conserved functions in cellular metabolism and chromatin regulation. Out of the seven human Sirtuins, the function of Sirt2 is the least understood. The purpose of the present study was to investigate the role of Sir2A, a homolog of human Sirt2 in Dictyostelium discoideum (Dd), a lower eukaryote. We created both overexpressing and deletion strains of Ddsir2A to analyse its functions. We observed sir2A mRNA expression throughout development and the transcript was present in the prespore/spore region of multicellular structures developed. They show a preference towards prestalk/stalk pathway when co-developed with wildtype cells during chimera formation. Deletion strain showed a multi-tipped phenotype, decrease in cell proliferation and inhibition of autophagy. In conclusion, our results show low cAMP levels, reduced cell-adhesion, weak cell migration and impaired autophagy to be responsible for the phenotype shown by the null cells. This study provides new insights into the functions of Ddsir2A.
Status: ppublish Type: Journal article Source: PUBMED PubMed ID: 29872023

 
Genes addressed in this paper
atg1 atg18 atg5 atg8a atg8b atg9 cadA csaA
Topics in this paper
Mammalian Gene Related







Non-mammalian Gene Related







Cellular Location







Function/Process







Transcriptional Regulation X X X X X X X X
RNA Levels and Processing X X X X X X X X
Cell Type Localization







Protein Physical Properties







Protein Functional Domain







Strains/Constructs







Development/Morphogenesis







Chemotaxis/Motility







Phylogenetic Analysis







Regulated By X X X X X X X X
Growth







Mutants/Phenotypes









Genes addressed in this paper
sir2A sir2B sir2C sir2D sir2E tipD
Topics in this paper
Mammalian Gene Related X X X X X
Non-mammalian Gene Related X X X X X
Cellular Location X




Function/Process X




Transcriptional Regulation




X
RNA Levels and Processing X



X
Cell Type Localization X




Protein Physical Properties X X X X X
Protein Functional Domain X X X X X
Strains/Constructs X




Development/Morphogenesis X




Chemotaxis/Motility X




Phylogenetic Analysis X X X X X
Regulated By




X
Growth X




Mutants/Phenotypes X