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Odell, Jacob, Sikirzhytski, Vitali, Tikhonenko, Irina, Cobani, Sonila, Khodjakov, Alexey, Koonce, Michael, (2019) ' Force balances between interphase centrosomes, as revealed by laser ablation. ' Mol. Biol. Cell mbcE19010034

 

Abstract:Numerous studies have highlighted the self-centering activities of individual microtubule arrays in animal cells, but relatively few works address the behavior of multiple arrays that coexist in a common cytoplasm. In multinucleated Dictyostelium discoideum cells, each centrosome organizes a radial microtubule network and these networks remain separate from one another. This feature offers an opportunity to reveal mechanism(s) responsible for positioning of multiple centrosomes. Using a laser microbeam to eliminate one of the two centrosomes in binucleate cells, we show that the unaltered array is rapidly repositioned at the cell center. This result demonstrates that each microtubule array is constantly subject to centering forces and infers a mechanism to balance the positions of multiple arrays. Our results address the limited actions of three kinesins and a crosslinking MAP that are known to have effects in maintaining microtubule organization, and suggest a simple means used to keep the arrays separated. Movie S1 Movie S1 Non-irradiated control binucleate WT cell demonstrating dynamics of centrosomes and interphase MTs. Frames recorded at 5-s intervals. Movie S2 Movie S2 Examples of centrosome/MT array movement following ablation of one of two centrosomes in binucleate cells. Frames recorded at 5-s intervals. Movie S3 Movie S3 Examples of centrosome/MT array movement following ablation of one of two centrosomes in binucleate cells. Frames recorded at 5-s intervals. Movie S4 Movie S4 Examples of centrosome/MT array movement following ablation of one of two centrosomes in binucleate cells. Frames recorded at 5-s intervals.
Status:	aheadofprint	Type:	Journal article	Source:	PUBMED	PubMed ID:	31067156

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	Genes addressed in this paper
	ase1A	kif10	kif8	kif9
Topics in this paper
Protein Physical Properties			X
Protein Functional Domain			X
Cytoskeleton	X	X	X	X
Strains/Constructs			X
Cellular Location			X
Mutants/Phenotypes	X	X	X	X
Techniques and Reagents	X	X	X	X
Function/Process			X