|Page Contents: Abstract|
Deichsel, H., Friedel, S., Detterbeck, A., Coyne, C., Hamker, U., MacWilliams, K., (1999)
' Green fluorescent proteins with short half-lives as reporters in Dictyostelium discoideum. '
Dev Genes Evol
|Abstract:We describe two modifications of the popular reporter green fluorescent protein (GFP) which have short half-lives in our system, the cellular slime mould Dictyostelium discoideum. One of these bears an N-terminal ubiquitin; this GFP was originally planned to be a substrate of the "N-end-rule" pathway, but deubiquitination does not seem to occur, and a degradation by the UFD (ubiquitin-fusion-degradation pathway seems more probable. The protein half-life is about 3-5 h. The second construct has an N-terminus derived from the L11 ribosomal protein; it is transported to the nucleus and broken down much more rapidly than the ubiquitin fusion (protein half-life about 30 min). We show examples of the use of these reporters in the study of gene expression in Dictyostelium.|
|Status:||Published||Type:||Journal article||Source:||PUBMED||PubMed ID:||9914420|