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Hinas, A., Larsson, P., Avesson, L., Kirsebom, A., Virtanen, A., Soderbom, F., (2006) ' Identification of the major spliceosomal RNAs in Dictyostelium discoideum reveals developmentally regulated U2 variants and polyadenylated snRNAs. ' Eukaryot Cell 5 924-34

 

Abstract:Most eukaryotic mRNAs depend upon precise removal of introns by the spliceosome, a complex of RNAs and proteins. Splicing of pre-mRNA is known to take place in Dictyostelium discoideum, and we previously isolated the U2 spliceosomal RNA experimentally. In this study, we identified the remaining major spliceosomal RNAs in Dictyostelium by a bioinformatical approach. Expression was verified from 17 small nuclear RNA (snRNA) genes. All these genes are preceded by a putative noncoding RNA gene promoter. Immunoprecipitation showed that snRNAs U1, U2, U4, and U5, but not U6, carry the conserved trimethylated 5' cap structure. A number of divergent U2 species are expressed in Dictyostelium. These RNAs carry the U2 RNA hallmark sequence and structure motifs but have an additional predicted stem-loop structure at the 5' end. Surprisingly, and in contrast to the other spliceosomal RNAs in this study, the new U2 variants were enriched in the cytoplasm and were developmentally regulated. Furthermore, all of the snRNAs could also be detected as polyadenylated species, and polyadenylated U1 RNA was demonstrated to be located in the cytoplasm.
Status:	Published	Type:	Journal article	Source:	PUBMED	PubMed ID:	16757740

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	Genes addressed in this paper
	rnu1a	rnu1b	rnu1c	rnu1d	rnu1e_ps	rnu2a	rnu2b	rnu2c
Topics in this paper
RNA Levels and Processing	X	X	X	X		X	X	X
DNA/RNA Sequence Features	X	X	X	X	X	X	X	X
Cellular Location	X	X				X	X	X
Synonym	X	X	X	X	X	X	X	X

	Genes addressed in this paper
	rnu2d	rnu2e	rnu2f	rnu2g	rnu4a	rnu4b	rnu4c	rnu5a
Topics in this paper
RNA Levels and Processing	X	X	X	X	X	X	X	X
DNA/RNA Sequence Features	X	X	X	X	X	X	X	X
Cellular Location		X	X	X	X	X	X	X
Synonym	X	X	X	X	X	X	X	X

	Genes addressed in this paper
	rnu5b	rnu6
Topics in this paper
RNA Levels and Processing	X	X
DNA/RNA Sequence Features	X	X
Cellular Location	X	X
Synonym	X	X